Physical exercise ameliorates the reduction of neural stem cell, cell proliferation and neuroblast differentiation in senescent mice induced by D-galactose

BMC Neuroscience

Yeo Sung Yoon¹  Je Kyung Seong¹  In Koo Hwang¹  Moo-Ho Won³  Hyo Young Jung¹  Woosuk Kim¹  Jung Hoon Choi²  Dae Won Kim⁴  Hee Sun Yim⁴  Dae Young Yoo¹  Jong Whi Kim¹  Sung Min Nam¹ 

[1] Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea;Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, South Korea;Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea;Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea

关键词: Mice;    Brain derived neurotrophic factor;    Phosphorylated cAMP-response element binding protein;    Adult neurogenesis;    Hippocampus;    Treadmill exercise;    D-galactose;   

Others  :  1090728
DOI  :  10.1186/s12868-014-0116-4

 received in 2013-07-19, accepted in 2014-10-06,  发布年份 2014

【 摘 要 】

Background

Aging negatively affects adult hippocampal neurogenesis, and exercise attenuates the age-related reduction in adult hippocampal neurogenesis. In the present study, we used senescent mice induced by D-galactose to examine neural stem cells, cell proliferation, and neuronal differentiation with or without exercise treatment. D-galactose (100 mg/kg) was injected to six-week-old C57BL/6 J mice for 6 weeks to induce the senescent model. During these periods, the animals were placed on a treadmill and acclimated to exercise for 1 week. Then treadmill running was conducted for 1 h/day for 5 consecutive days at 10-12 m/min for 5 weeks.

Results

Body weight and food intake did not change significantly after D-galactose administration with/without treadmill exercise, although body weight and food intake was highest after treadmill exercise in adult animals and lowest after treadmill exercise in D-galactose-induced senescent model animals. D-galactose treatment significantly decreased the number of nestin (a neural stem cell marker), Ki67 (a cell proliferation marker), and doublecortin (DCX, a differentiating neuroblast marker) positive cells compared to those in the control group. In contrast, treadmill exercise significantly increased Ki67- and DCX-positive cell numbers in both the vehicle- and D-galactose treated groups. In addition, phosphorylated cAMP-response element binding protein (pCREB) and brain derived neurotrophic factor (BDNF) was significantly decreased in the D-galactose treated group, whereas exercise increased their expression in the subgranular zone of the dentate gyrus in both the vehicle- and D-galactose-treated groups.

Conclusion

These results suggest that treadmill exercise attenuates the D-galactose-induced reduction in neural stem cells, cell proliferation, and neuronal differentiation by enhancing the expression of pCREB and BDNF in the dentate gyrus of the hippocampus.

【 授权许可】

   
2014 Nam et al.; licensee BioMed Central Ltd.

【 图 表 】

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