Temperature during early development has long-term effects on microRNA expression in Atlantic cod

BMC Genomics

Igor Babiak³  Hilde Toften¹  Velmurugu Puvanendran¹  Steinar D Johansen²  Teshome Tilahun Bizuayehu³ 

[1] Nofima AS, Muninbakken 9-13, Tromsø, 9291, NO, Norway;Arctic University of Norway, FHS, RNA Lab, Dept Med Biol, Tromsø, N-9037, Norway;University of Nordland, Faculty of Biosciences and Aquaculture, Bodø, 8049, Norway

关键词: Thermal plasticity;    miRNA;    Methylation;    Embryonic development;    Atlantic cod;   

Others  :  1173970
DOI  :  10.1186/s12864-015-1503-7

 received in 2015-03-06, accepted in 2015-03-27,  发布年份 2015

【 摘 要 】

Background

Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile.

Results

We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.

Conclusions

Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

【 授权许可】

   
2015 Bizuayehu et al.; licensee BioMed Central.

【 图 表 】

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