Immune response to Propionibacterium acnes in patients with sarcoidosis – in vivo and in vitro

BMC Pulmonary Medicine

Antje Prasse⁵  Marina A Freudenberg³  Joachim Müller-Quernheim⁴  Peggy Engelhard²  Niklas Lützen¹  Sandrine Tchaptchet³  Jonas Christian Schupp⁴ 

[1]Department of Radiology, University Medical Centre, Freiburg, Germany

[2]Faculty of Biology, University of Freiburg, Freiburg, Germany

[3]Department of Developmental Immunology, Max Planck Institute of Immunobiology und Epigenetics, Freiburg, Germany

[4]Department of Pneumology, University Medical Centre, Albert-Ludwigs University, Killianstr. 5, Freiburg, 79106, Germany

[5]Department of Pneumology, Medical School, Hannover, Germany

关键词: Immune response;    Immunoglobulin;    Propionibacterium acnes;    human;    Sarcoidosis;   

Others  :  1222577
DOI  :  10.1186/s12890-015-0070-7

 received in 2015-03-30, accepted in 2015-07-06,  发布年份 2015

【 摘 要 】

Background

Propionibacterium acnes was found in lungs and lymph nodes of patients with sarcoidosis and may induce hypersensitivity type granuloma formation. Data regarding the immune response to P. acnes of European sarcoid patients are scarce.

Methods

We assessed the total IgG and IgA amount and specific antibodies to P. acnes and to Staphylococcus aureus, serving as a control, in BAL fluid of 64 patients with sarcoidosis and of 21 healthy volunteers. In a subcohort of sarcoid patients and controls, TNF-α and GM-CSF production of BAL cells stimulated with heat-killed P. acnes were measured.

Results

In sarcoid patients, the total IgG and IgA levels in BAL fluid were significantly elevated compared to healthy volunteers. IgG and IgA titres against P. acnes and S. aureus were increased in sarcoid patients, yet based on the total amount of antibodies, only antibodies directed against P. acnes were relatively and significantly increased. Furthermore, BAL cells of sarcoid patients produced significantly more TNF-α and GM-CSF upon stimulation with heat-killed P. acnes compared to controls.

Conclusions

Patients with sarcoidosis had elevated levels of specific antibodies against P. acnes which suggest contact with this bacterium in the past. Furthermore, BAL cells of sarcoid patients produced inflammatory cytokines (TNF-α and GM-CSF) upon stimulation with P. acnes indicating potential involvement of this pathogen in the pathogenesis of sarcoidosis in some patients.

【 授权许可】

   
2015 Schupp et al.

【 图 表 】

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