T 1ρ magnetic resonance imaging quantification of early articular cartilage degeneration in a rabbit model

BMC Musculoskeletal Disorders

Meng Chen¹  Fei Wang¹  Jing Zhang¹  Hao Wang²  Si Shen¹ 

[1] Medical Imaging Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;Pain Clinic, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China

关键词: Histology;    Proteoglycan;    Early cartilage degeneration;    Magnetic resonance imaging;    T1ρ;   

Others  :  1233980
DOI  :  10.1186/s12891-015-0810-0

 received in 2015-09-05, accepted in 2015-11-10,  发布年份 2015

【 摘 要 】

Background

Osteoarthritis (OA) is a serious problem in the recent aging society, and early diagnosis and intervention of articular cartilage degeneration are very important for the onset of OA. Therefore, development of newer MRI techniques is necessary and expected for detection of early articular cartilage degeneration.

Methods

24 rabbits were randomly divided into four equal experimental groups (Group A, B, C, D) to establish articular cartilage models in different grades of early degeneration by injecting papain into the left knee joint cavity. Another 8 rabbits were considered as blank control (Group E), and then randomized into four subgroups (E _A , E _B , E _C , E _D ). T _1ρand T ₂ -weighted images of the bilateral knee joints were obtained for rabbits by using 3.0 T MRI. Group A, B, C, and D were imaged respectively at 1, 2, 3, and 4 weeks post-operation, and E _A , E _B , E _C , E _Dunderwent the same period imaging. Rabbits were sacrificed after scanning and the femoral condyle cartilage (FCC) was histological examined. T _1ρvalues of the femoral condyle cartilage were measured and statistically analyzed, and contrasted with the histologic results.

Results

T _1ρvalues of the left side in experimental groups were significantly higher than the right side (P < 0.05), and which increased gradually with the passage of post-operation time (P < 0.05). Histological examination demonstrated the proteoglycan content of the left side decreased, and indicated the occurrence of early degeneration.

Conclusions

T _1ρMRI can sensitively and quantitatively reflect the change in proteoglycans prior to the morphologic alterations of articular cartilage, and T _1ρvalue is gradually increased with a decrease in proteoglycan content, therefore that T _1ρcould potentially act as a reliable tool to identify early cartilage degeneration.

【 授权许可】

   
2015 Shen et al.

【 图 表 】

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