Quantitative detection of free 24S-hydroxycholesterol, and 27-hydroxycholesterol from human serum

BMC Neuroscience

Norman J Haughey¹  Veera Venkata Ratnam Bandaru² 

[1] Department of Psychiatry, Division of Geriatric Psychiatry and Neuropsychiatry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA;Department of Neurology, Richard T. Johnson Division of Neuroimmunology and Neurological Infections, The Johns Hopkins University School of Medicine, Carnegie 616A, 600 North Wolfe Street, Baltimore 21287, MD, USA

关键词: LC/ESI/MS/MS;    27-hydroxycholesterol;    24S-hydroxycholesterol;    Serum;    HIV associated neurocogntiive disorder;    Multiple sclerosis;    Alzheimer’s;   

Others  :  1090240
DOI  :  10.1186/s12868-014-0137-z

 received in 2014-06-20, accepted in 2014-12-17,  发布年份 2014

【 摘 要 】

Background

Cholesterol metabolism is important for the maintenance of myelin and neuronal membranes in the central nervous system. Blood concentrations of the brain specific cholesterol metabolite 24S-hydroxysterol to the peripheral metabolite 27-hydroxycholesterol may be useful surrogate markers for neurodegenerative diseases including Alzheimer’s disease, Huntington’s disease, HIV-Associated Neurocognitive Disorders, and Multiple Sclerosis. However, current methods to isolate hydroxycholesterols are labor intensive, prone to produce variable extraction efficiencies and do not discriminate between free and esterfied forms of hydroxycholesterols. Since free hydroxycholesterols are the biologically active form of these sterols, separating free from esterfied forms may provide a sensitive measure to identify disease-associated differences in brain sterol metabolism.

Results

We found that average human serum concentrations were 12.3 ± 4.79 ng/ml for free 24(s)-hydroxycholesterol and 17.7 ± 8.5 ng/ml for 27-hydroxycholesterol.

Conclusion

Serum measurements of these biologically active oxysterols may be useful surrogate measures for brain health in a variety of neurodegenerative conditions.

【 授权许可】

   
2014 Bandaru and Haughey; licensee BioMed Central.

【 图 表 】

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