Complete sequencing of Novosphingobium sp. PP1Y reveals a biotechnologically meaningful metabolic pattern

BMC Genomics

Alberto Di Donato²  Francesco Salvatore³  Giovanni Paolella⁵  Luca Troncone²  Lorenzo Durante²  Luca Cozzuto¹  Barbara Naso⁵  Viviana Izzo⁴  Valeria Cafaro²  Piergiuseppe Cantiello¹  Mauro Petrillo⁵  Eugenio Notomista²  Valeria D’Argenio⁵ 

[1] CEINGE-Biotecnologie Avanzate, Napoli, Italy;Dipartimento di Biologia, Università di Napoli Federico II, Napoli, Italy;IRCCS-Fondazione SDN, Naples, Italy;Dipartimento di Medicina e Chirurgia, Università degli Studi di Salerno, Salerno, Italy;Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy

关键词: Aromatic pollutant compounds/bioremediation;    Next generation sequencing;    Sphingomonads;    Novosphingobium sp. PP1Y;    De novo sequencing;   

Others  :  1217214
DOI  :  10.1186/1471-2164-15-384

 received in 2013-11-05, accepted in 2014-05-08,  发布年份 2014

【 摘 要 】

Background

Novosphingobium sp. strain PP1Y is a marine α-proteobacterium adapted to grow at the water/fuel oil interface. It exploits the aromatic fraction of fuel oils as a carbon and energy source. PP1Y is able to grow on a wide range of mono-, poly- and heterocyclic aromatic hydrocarbons. Here, we report the complete functional annotation of the whole Novosphingobium genome.

Results

PP1Y genome analysis and its comparison with other Sphingomonadal genomes has yielded novel insights into the molecular basis of PP1Y’s phenotypic traits, such as its peculiar ability to encapsulate and degrade the aromatic fraction of fuel oils. In particular, we have identified and dissected several highly specialized metabolic pathways involved in: (i) aromatic hydrocarbon degradation; (ii) resistance to toxic compounds; and (iii) the quorum sensing mechanism.

Conclusions

In summary, the unraveling of the entire PP1Y genome sequence has provided important insight into PP1Y metabolism and, most importantly, has opened new perspectives about the possibility of its manipulation for bioremediation purposes.

【 授权许可】

   
2014 D’Argenio et al.; licensee BioMed Central Ltd.

【 图 表 】

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