Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies

BMC Genomics

Serge Laberge³  Hani Antoun⁴  Linda Harris¹  Anne Johnston¹  Abdollah-Fardin Ghobakhlou² 

[1]Eastern Cereal & Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa K1A 0C6, Ontario, Canada

[2]Graduate Programs in Agri-Food Microbiology, Faculty of Agriculture and Food Sciences, Laval University, Quebec City G1V 0A6, Quebec, Canada

[3]Soils and Crops Research Development Center, Agriculture and Agri-Food Canada, Quebec City G1V 2 J3, Quebec, Canada

[4]Department of Soils and Agri-Food Engineering, Laval University, Quebec City G1V 0A6, Quebec, Canada

关键词: Arctic Mesorhizobium;    Nitrogen fixation;    Cold adaptation;    Quantitative PCR;    Transcriptomics;    Gene expression;    Microarray;    Genomic library;    α-proteobacteria;   

Others  :  1203960
DOI  :  10.1186/s12864-015-1611-4

 received in 2014-11-10, accepted in 2015-05-01,  发布年份 2015

【 摘 要 】

Background

Arctic Mesorhizobium strain N33 was isolated from nodules of the legume Oxytropis arctobia in Canada’s eastern Arctic. This symbiotic bacterium can grow at temperatures ranging from 0 to 30 °C, fix nitrogen at 10 °C, and is one of the best known cold-adapted rhizobia. Despite the economic potential of this bacterium for northern regions, the key molecular mechanisms of its cold adaptation remain poorly understood.

Results

Using a microarray printed with 5760 Arctic Mesorhizobium genomic clones, we performed a partial transcriptome analysis of strain N33 grown under eight different temperature conditions, including both sustained and transient cold treatments, compared with cells grown at room temperature. Cells treated under constant (4 and 10 °C) low temperatures expressed a prominent number of induced genes distinct from cells treated to short-term cold-exposure (<60 min), but exhibited an intermediate expression profile when exposed to a prolonged cold exposure (240 min). The most prominent up-regulated genes encode proteins involved in metabolite transport, transcription regulation, protein turnover, oxidoreductase activity, cryoprotection (mannitol, polyamines), fatty acid metabolism, and membrane fluidity. The main categories of genes affected in N33 during cold treatment are sugar transport and protein translocation, lipid biosynthesis, and NADH oxidoreductase (quinone) activity. Some genes were significantly down-regulated and classified in secretion, energy production and conversion, amino acid transport, cell motility, cell envelope and outer membrane biogenesis functions. This might suggest growth cessation or reduction, which is an important strategy to adjust cellular function and save energy under cold stress conditions.

Conclusion

Our analysis revealed a complex series of changes associated with cold exposure adaptation and constant growth at low temperatures. Moreover, it highlighted some of the strategies and different physiological states that Mesorhizobium strain N33 has developed to adapt to the cold environment of the Canadian high Arctic and has revealed candidate genes potentially involved in cold adaptation.

【 授权许可】

   
2015 Ghobakhlou et al.; licensee BioMed Central.

【 图 表 】

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Figure 1.

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