A comprehensive proteomic approach to identifying capacitation related proteins in boar spermatozoa

BMC Genomics

Myung-Geol Pang²  Seongsoo Hwang¹  Young-Ah You²  Yoo-Jin Park²  Sung-Jae Yoon²  Jin Kim²  June-Sub Lee²  Md Saidur Rahman²  Woo-Sung Kwon² 

[1] Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi-do 441-706, Republic of Korea;Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea

关键词: 2DE;    Spermatozoa;    Boar;    Proteomics;    Capacitation;   

Others  :  1128459
DOI  :  10.1186/1471-2164-15-897

 received in 2014-05-09, accepted in 2014-10-06,  发布年份 2014

【 摘 要 】

Background

Mammalian spermatozoa must undergo capacitation, before becoming competent for fertilization. Despite its importance, the fundamental molecular mechanisms of capacitation are poorly understood. Therefore, in this study, we applied a proteomic approach for identifying capacitation-related proteins in boar spermatozoa in order to elucidate the events more precisely. 2-DE gels were generated from spermatozoa samples in before- and after-capacitation. To validate the 2-DE results, Western blotting and immunocytochemistry were performed with 2 commercially available antibodies. Additionally, the protein-related signaling pathways among identified proteins were detected using Pathway Studio 9.0.

Result

We identified Ras-related protein Rab-2, Phospholipid hydroperoxide glutathione peroxidase (PHGPx) and Mitochondrial pyruvate dehydrogenase E1 component subunit beta (PDHB) that were enriched before-capacitation, and NADH dehydrogenase 1 beta subcomplex 6, Mitochondrial peroxiredoxin-5, (PRDX5), Apolipoprotein A-I (APOA1), Mitochondrial Succinyl-CoA ligase [ADP-forming] subunit beta (SUCLA2), Acrosin-binding protein, Ropporin-1A, and Spermadhesin AWN that were enriched after-capacitation (>3-fold) by 2-DE and ESI-MS/MS. SUCLA2 and PDHB are involved in the tricarboxylic acid cycle, whereas PHGPx and PRDX5 are involved in glutathione metabolism. SUCLA2, APOA1 and PDHB mediate adipocytokine signaling and insulin action. The differentially expressed proteins following capacitation are putatively related to sperm functions, such as ROS and energy metabolism, motility, hyperactivation, the acrosome reaction, and sperm-egg interaction.

Conclusion

The results from this study elucidate the proteins involved in capacitation, which may aid in the design of biomarkers that can be used to predict boar sperm quality.

【 授权许可】

   
2014 Kwon et al.; licensee BioMed Central Ltd.

【 图 表 】

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