Large-scale transcriptome comparison reveals distinct gene activations in wheat responding to stripe rust and powdery mildew

BMC Genomics

Wanquan Ji¹  Xinlun Liu¹  Yingbin Nie¹  Yajuan Wang¹  Ying Fu¹  Hao Li¹  Min Liu¹  Changyou Wang¹  Yongzheng Yang¹  Hong Zhang¹ 

[1] State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, Shaanxi 712100, China

关键词: Gene expression;    RNA-Seq;    Powdery mildew;    Stripe rust;    Bread wheat;   

Others  :  1128458
DOI  :  10.1186/1471-2164-15-898

 received in 2014-07-18, accepted in 2014-10-09,  发布年份 2014

【 摘 要 】

Background

Stripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis f. sp. tritici; Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the host defense response because both pathogens are biotrophic fungi. The main objective of our study was to identify co-regulated mRNAs that show a change in expression pattern after inoculation with Pst or Bgt, and to identify mRNAs specific to the fungal stress response.

Results

The transcriptome of the hexaploid wheat line N9134 inoculated with the Chinese Pst race CYR 31 was compared with that of the same line inoculated with Bgt race E09 at 1, 2, and 3 days post-inoculation. Infection by Pst and Bgt affected transcription of 23.8% of all T. aestivum genes. Infection by Bgt triggered a more robust alteration in gene expression in N9134 compared with the response to Pst infection. An array of overlapping gene clusters with distinctive expression patterns provided insight into the regulatory differences in the responses to Bgt and Pst infection. The differentially expressed genes were grouped into seven enriched Kyoto Encyclopedia of Genes and Genomes pathways in Bgt-infected leaves and four pathways in Pst-infected leaves, while only two pathways overlapped. In the plant–pathogen interaction pathway, N9134 activated a higher number of genes and pathways in response to Bgt infection than in response to Pst invasion. Genomic analysis revealed that the wheat genome shared some microbial genetic fragments, which were specifically induced in response to Bgt and Pst infection.

Conclusions

Taken together, our findings indicate that the responses of wheat N9134 to infection by Bgt and Pst shows differences in the pathways and genes activated. The mass sequence data for wheat–fungus interaction generated in this study provides a powerful platform for future functional and molecular research on wheat–fungus interactions.

【 授权许可】

   
2014 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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