Mannosylated lipoarabinomannan in serum as a biomarker candidate for subclinical bovine tuberculosis

BMC Research Notes

Srinand Sreevatsan³  Tyler Thacker¹  Wade Ray Waters¹  João Ribeiro-Lima²  Elise A Lamont⁴ 

[1] United States Department of Agriculture, National Animals Disease Center, Ames, Iowa, USA;Faculdade de Medicina Veterinária, Universidade Lusósona de Humanidades e Tecnologias, Lisboa, Portugal;Veterinary Population Medicine and Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, 55108 St. Paul, MN, USA;Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA

关键词: Serum;    Subclinical Infection;    Diagnostics;    Lipoarabinomannan;    Cattle;    Mycobacterium bovis;    Biomarker;    Bovine tuberculosis;   

Others  :  1130261
DOI  :  10.1186/1756-0500-7-559

 received in 2014-04-25, accepted in 2014-08-18,  发布年份 2014

【 摘 要 】

Background

Early and unambiguous detection of bovine tuberculosis (bTB), a significant disease of cattle worldwide, is necessary to control the spread of infection to other animals and humans. Current testing strategies are laborious, time consuming and heavily reliant on host responses that do not distinguish bTB from other mycobacteria. We report the presence of a pathogen signature, liparabinomannan (LAM), as a potential biomarker for bTB infection.

Findings

Fifty-five animals (uninfected [n = 33], bTb [n = 10] and exposed cases [n = 12]) from a well characterized bovine serum repository were screened for the presence of LAM using a commercially available ELISA. Analysis showed that LAM had a sensitivity of 100% and a specificity of 91.7% for bTB detection (bTB positive versus bTB exposed animals).

Conclusion

LAM detection easily separated bTB infected animals from bTB exposed and negative controls. We propose that pathogen related markers, such as LAM, should be included with current testing strategies as a battery diagnostic for bTB.

【 授权许可】

   
2014 Lamont et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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