Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA

BMC Microbiology

Andrew Jenkins⁵  Snorre Stuen¹  Andreas Matussek²  Bjørn-Erik Kristiansen⁴  Dag Hvidsten³  Anna J. Henningsson² 

[1] Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway;Department of Clinical Microbiology, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden;Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway;Research group of host-microbe interactions, Department of Medical Biology, Faculty of Health Sciences, UiT - The Arctic University of Norway, Tromsø, Norway;Department of Environmental and Health Studies, Telemark University College, Bø, Norway

关键词: Prevalence;    gltA;    Norway;    TaqMan realtime PCR;    Ixodes ricinus;    Anaplasma phagocytophilum;   

Others  :  1227608
DOI  :  10.1186/s12866-015-0486-5

 received in 2015-03-03, accepted in 2015-07-16,  发布年份 2015

【 摘 要 】

Background

A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96).

Results

Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies.

Conclusion

This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.

【 授权许可】

   
2015 Henningsson et al.

【 图 表 】

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