Serum neuron specific enolase – impact of storage and measuring method

BMC Research Notes

Anders Isaksson¹  Hans Friberg⁴  Tobias Cronberg³  Malin Rundgren² 

[1] Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, Lund, Sweden;Department of Intensive and Perioperative Care, Skane University Hospital, Lund, 221 85 Lund, Sweden;Department of Clinical Sciences, Division of Neurology, Lund University, Lund, Sweden;Center For Resuscitation Science, Skane University Hospital, Lund, Sweden

关键词: Prognostication;    NSE;    Neuron specific enolase;    Methodology;    Hypothermia;    Cardiac arrest;    Biomarkers;   

Others  :  1127239
DOI  :  10.1186/1756-0500-7-726

 received in 2014-09-11, accepted in 2014-10-03,  发布年份 2014

【 摘 要 】

Background

Neuron specific enolase (NSE) is a recognized biomarker for assessment of neurological outcome after cardiac arrest, but its reliability has been questioned. Our aim was to investigate what influence storage of samples and choice of measuring methods may have on levels of NSE in peripheral blood.

Methods

Two serum samples were drawn simultaneously from 51 hypothermia treated cardiac arrest patients. One sample (original sample) was analysed when collected, using the Diasorin-method (LIAISON®NSE, LNSE). The other sample was frozen, stored at −70°C (stored sample), and reanalysed in the same laboratory 4–7 years later using both the Diasorin method and a Roche-method (NSE Cobas e601, CNSE). In addition, a comparison of the two methods was performed on 29 fresh samples.

Results

The paired NSE results in original and stored samples were not significantly different, using the LNSE-method. The two methods produced significantly different results (p < 0.0001) on the paired, stored samples, with the CNSE method yielding higher values than the LNSE-method in 96% of samples. The CNSE method resulted in 36% higher values on average. In the method comparison on fresh samples, the CNSE-method generated on average 15% higher values compared to the LNSE-method, and the difference between the paired results was significant (p < 0.0001).

Conclusion

The CNSE method generated consistently higher NSE-values than the LNSE method and this difference was more pronounced when frozen samples were analysed. Tolerability for prolonged freezing was acceptable.

【 授权许可】

   
2014 Rundgren et al.; licensee BioMed Central Ltd.

【 图 表 】

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