Multilocus sequence types of invasive pneumococcal isolates from Danish infants (0–90 days) 2003–2013

BMC Research Notes

Steen Hoffmann¹  Tine Dalby¹  Hans-Christian Slotved¹ 

[1] Neisseria and Streptococcus Reference Laboratory, Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, Copenhagen, 2300, Denmark

关键词: Streptococcus pneumoniae;    MLST;    IPD isolates;    Invasive pneumococcal disease;    Infants;   

Others  :  1229561
DOI  :  10.1186/s13104-015-1540-y

 received in 2015-04-10, accepted in 2015-10-05,  发布年份 2015

【 摘 要 】

Background

A pneumococcal conjugate vaccine (PCV) has been part of the Danish childhood immunization programme since October 2007. It is administered at the ages of 3, 5 and 12 months and healthy infants younger than 90 days are consequently not vaccinated. Initially the PCV-7 vaccine was used but this was replaced by the PCV-13 in April 2010. Vaccination coverage in Denmark is approximately 90 %.The aim of this study was to present multilocus sequence typing (MLST) profiles of Streptococcus pneumoniae isolates from Danish infants (0–90 days) with invasive pneumococcal disease (IPD) in the period 2003–2013.

Findings

32 IPD isolates were investigated for their MLST profiles. The identified sequence types (STs) had previously been observed in other European countries. Among the clones were ST 306 (serotype 1), ST 180 (serotype 3) and ST 191 (serotype 7F).

Conclusions

The ST profile distribution in this study is similar to that observed in other European studies and show a variety of STs. Our data show that the majority of STs found in Denmark is also observed in other European countries, indicating that the IPD isolates were from clones generally circulating in Europe.

【 授权许可】

   
2015 Slotved et al.

【 参考文献 】

• [1]Howitz M, Hartvig-Christiansen A, Harboe ZB, Mølbak K: Surveillance of bacterial meningitis in children under 2 y of age in Denmark, 1997–2006. Scand J Infect Dis 2008, 40:881-887.
• [2]O’Brien KL: PCV13 impact evaluations: the obvious and the unpredicted. Pediatr Infect Dis J. 2002, 32:264-265.
• [3]Feavers I, Knezevic I, Powell M, Griffiths EWHO: WHO consultation on serological criteria for evaluation and licensing of new pneumococcal vaccines. Challenges in the evaluation and licensing of new pneumococcal vaccines, 7–8, Ottawa, Canada. Vaccine. 2008, 2009(27):3681-3688.
• [4]Borrow R, Heath PT, Siegrist CA: Use of pneumococcal polysaccharide vaccine in children: what is the evidence? Curr Opin Infect Dis. 2012, 25:292-303.
• [5]Harboe ZB, Dalby T, Weinberger DM, Benfield T, Mølbak K, Slotved HC, et al.: Impact of 13-valent pneumococcal conjugate vaccination in invasive pneumococcal disease incidence and mortality. Clin Infect Dis 2014, 59:1066-1073.
• [6]Valentiner-Branth P, Bjerre CC, Andersen PH, Lambertsen L, Ingels H, Hoffmann S, Konradsen HB. PCV7 coverage and invasive pneumococcal disease 2011. EPI-NEWS week 21, 2012. http://www.ssi.dk/English/News/EPI-NEWS/2012/. Accessed 8 April 2015.
• [7]Simell B, Auranen K, Käyhty H, Goldblatt D, Dagan R, O’Brien KL: The fundamental link between pneumococcal carriage and disease. Expert Rev Vaccines. 2012, 11:841-855.
• [8]Slotved HC, Dalby T, Hoffmann S: Invasive pneumococcal isolates from Danish infants (0–90 Days) during the years 1943 to 2013. PLoS One 2014, 9(8):e106180.
• [9]Lambertsen L, Brendstrup M, Friis H, Christensen JJ: Molecular characterization of invasive penicillin non-susceptible Streptococcus pneumoniae from Denmark, 2001 to 2005. Scand J Infect Dis 2010, 42:333-340.
• [10]Feil EJ, Li BC, Aanensen DM, Hanage WP, Spratt BG: eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 2004, 186:1518-1530.
• [11]Loman NJ, Gladstone RA, Constantinidou C, Tocheva AS, Jefferies JM, Faust SN, et al.: Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. PLoS One 2013, 8:e64731.
• [12]Donkor ES, Adegbola RA, Wren BW, Antonio M: Population biology of Streptococcus pneumoniae in West Africa: multilocus sequence typing of serotypes that exhibit different predisposition to invasive disease and carriage. PLoS One 2013, 8:e53925.
• [13]Ardanuy C, Marimón JM, Calatayud L, Giménez M, Alonso M, Grau I, et al.: Epidemiology of invasive pneumococcal disease in older people in Spain (2007–2009): implications for future vaccination strategies. PLoS One 2012, 7:e43619.
• [14]Pichon B, Ladhani SN, Slack MP, Segonds-Pichon A, Andrews NJ, Waight PA, et al.: Changes in molecular epidemiology of Streptococcus pneumoniae causing meningitis following introduction of pneumococcal conjugate vaccination in England and Wales. J Clin Microbiol 2013, 51:820-827.
• [15]Vestrheim DF, Høiby EA, Aaberge IS, Caugant DA: Phenotypic and genotypic characterization of Streptococcus pneumoniae strains colonizing children attending day-care centers in Norway. J Clin Microbiol 2008, 46:2508-2518.