Kolaviron, a Garcinia biflavonoid complex ameliorates hyperglycemia-mediated hepatic injury in rats via suppression of inflammatory responses

BMC Complementary and Alternative Medicine

Oluwafemi O Oguntibeju¹  Nicole L Brooks²  Novel N Chegou³  Omolola R Ayepola¹ 

[1] Department of Biomedical Sciences, Cape Peninsula, Oxidative Stress Research Centre, University of Technology, Bellville, South Africa;Department of Wellness Sciences, Cape Peninsula University of Technology, Cape Town, South Africa;Department of Biomedical Sciences, Centre of Excellence for Biomedical Tuberculosis Research and MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Stellenbosch University, Tygerberg, South Africa

关键词: Chemotactic protein;    Pro-inflammatory cytokine;    Kolaviron;    Hepatic injury;    Diabetes;   

Others  :  1220433
DOI  :  10.1186/1472-6882-13-363

 received in 2013-09-09, accepted in 2013-12-11,  发布年份 2013

【 摘 要 】

Background

Chronic inflammation plays a crucial role in hyperglycemia-induced liver injury. Kolaviron (KV), a natural biflavonoid from Garcinia kola seeds have been shown to possess anti- inflammatory properties which has not been explored in diabetes. To our knowledge, this is the first study to investigate the effect of KV on pro-inflammatory proteins in the liver of diabetic rats.

Methods

Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg) in male Wistar rats. Kolaviron (100 mg/kg) was administered orally five times a week for six weeks. The concentrations of cytokines and chemokine were measured using Bio-plex Pro™ magnetic bead-based assays (Bio-Rad Laboratories, Hercules, USA). Plasma glucose and serum biomarkers of liver dysfunction were analyzed with diagnostic kits in an automated clinical chemistry analyzer. Insulin concentration was estimated by radioimmunoassay (RIA).

Result

Kolaviron (100mg/kg) treatment significantly ameliorated hyperglycemia and liver dysfunction. Serum levels of hepatic marker enzymes were significantly reduced in kolaviron treated diabetic rats. Kolaviron prevented diabetes induced increase in the hepatic levels of proinflammatory cytokines; interleukin (IL)-1beta, IL-6, tumour necrosis factor (TNF-α) and monocyte chemotactic protein (MCP-1).

Conclusion

The results of this study demonstrate that the hepatoprotective effects of kolaviron in diabetic rats may be partly associated with its modulating effect on inflammatory responses.

【 授权许可】

   
2013 Ayepola et al.; licensee BioMed Central Ltd.

【 图 表 】

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