Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis

BMC Research Notes

Olin E. Rhodes⁴  Kenneth L. Jones¹  Thomas W. McAuliffe²  Bruce A. Kimball³  Rochelle R. Beasley⁴  Stacey L. Lance⁴  Erin F. Abernethy⁴  Shem D. Unger⁴ 

[1]Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora 80045, CO, USA

[2]United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 210 Amau’ulu Road, Hilo 96720, HI, USA

[3]United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Resources Center, Monell Chemical Senses Center, 3500 Market Street, Philadelphia 19104, PA, USA

[4]Savannah River Ecology Laboratory, University of Georgia, Aiken 29802, SC, USA

关键词: Microsatellites;    Illumina;    Invasive species;    Brown tree snake;    Boiga irregularis;   

Others  :  1232736
DOI  :  10.1186/s13104-015-1620-z

 received in 2015-06-11, accepted in 2015-10-26,  发布年份 2015

【 摘 要 】

Background

Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust population genetic studies on Guam.

Findings

We isolated and characterized 33 microsatellite loci for the brown tree snake, B. irregularis. The loci were screened across 32 individuals from Guam. The number of alleles per locus ranged from three to ten, with an average of 4.62. The expected (H _e ) and observed heterozygosity (H _o ) ranged from 0.294 to 0.856 and from 0.031 to 0.813, with an average of 0.648 and 0.524, respectively. Significant deviations from Hardy–Weinberg equilibrium were detected at seven loci after Bonferoni correction. Probability of identity values ranged from 0.043 to 0.539.

Conclusions

These genetic markers are useful for understanding a suite of post-invasion population genetic parameters, sources of invasions, and effectiveness of management strategies for this invasive species.

【 授权许可】

   
2015 Unger et al.

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