Progesterone-induced changes in the phosphoryl potential during the meiotic divisions in amphibian oocytes: Role of Na/K-ATPase

BMC Developmental Biology

Raj K Gupta²  Adele B Kostellow²  Terry L Dowd¹  Gene A Morrill² 

[1] Department of Chemistry, Brooklyn College of the City University of New York Brooklyn, New York 11210, USA;Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA

关键词: bioenergetics;    oocytes;    meiosis;    Na/K-ATPase;    phosvitin;    ³¹P-NMR;   

Others  :  1087085
DOI  :  10.1186/1471-213X-11-67

 received in 2011-08-08, accepted in 2011-11-06,  发布年份 2011

【 摘 要 】

Background

Progesterone triggers resumption of the first meiotic division in the Rana pipiens oocyte by binding to the N-terminal external loop of the catalytic subunit of Na/K-ATPase, releasing a cascade of lipid second messengers. This is followed by internalization of specific membrane proteins, plasma membrane depolarization and nuclear membrane breakdown, culminating in arrest at second metaphase.

Results

Progesterone initiates an increase in phosphoryl potential during the first meiotic division, resulting in the accumulation of high energy protein phosphate by second metaphase arrest. ³¹P-NMR, with saturation transfer, demonstrates that the phosphocreatine level rises ~2 fold and that the "pseudo" first order rate constant for the creatine kinase reaction falls to ~20% of the control by the onset of nuclear membrane breakdown. ³²PO₄ pulse-labeling reveals a net increase in phosphorylation of yolk protein phosvitin during this period. The increased yolk protein phosphorylation coincides with internalization of membrane Na/K-ATPase and membrane depolarizatio

Conclusions

These results indicate that progesterone binding to the catalytic subunit of the Na-pump diverts ATP from cation regulation at the plasma membrane to storage of high energy phosphate in yolk protein. Phosvitin serves as a major energy source during fertilization and early cleavage stages and is also a storage site for cations (e.g. Na⁺, K⁺, Ca²⁺, Fe^2+/3+) essential for embryonic development.

【 授权许可】

   
2011 Morrill et al; licensee BioMed Central Ltd.

【 图 表 】

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