Novel monoclonal antibodies against Plasmodium falciparum histidine-rich protein 2: development and application in rapid diagnostic tests of malaria in hyperendemic regions of China and Myanmar

BMC Microbiology

Hui Liu²  Xiaochun Luo³  Mingquan Xie³  Wenmei Li⁴  Emmanuel E Dzakah¹  Keren Kang⁴ 

[1]Department of Molecular Biology and Biotechnology, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana

[2]Yunnan Provincial Center for Parasitic Diseases Control and Prevention (NIPD), Yunnan 650000, China

[3]School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China

[4]National Engineering Laboratory of Point-of-Care Tests, Guangzhou Wondfo Biotech Co. Ltd, Guangzhou 510663, China

关键词: Immunochromatographic test;    Point-of-care test;    Monoclonal antibodies;    Histidine-rich protein 2 exon II;    Malaria;   

Others  :  1212045
DOI  :  10.1186/s12866-015-0429-1

 received in 2014-10-23, accepted in 2015-04-22,  发布年份 2015

【 摘 要 】

Background

Malaria presents a considerable threat to public health. Histidine-rich protein 2 (HRP 2) is the major protein released into human blood upon infection by Plasmodium falciparum. In this study, we aimed to evaluate the immunogenicity of HRP 2 exon II and the efficacy of novel monoclonal antibodies (mAbs) against HRP 2 for Point-of-Care Test (POCT).

Methods

The recombinant protein was expressed in soluble form in E. coli and used to immunize mice for mAb production. Two IgG1 mAbs (1A5 and 1C10) with high affinity, specificity and sensitivity for both native and recombinant HRP 2 were selected after fusion of mouse spleen with myeloma cells. The affinity constant of 1A5 and 1C10 were 7.15 and 4.91 × 10-7 L/mol, respectively. Subsequently, an immunochromatograhic assay was used for screening of clinical samples in endemic regions of China and Myanmar.

Results

The immunochromatographic test retrospectively showed an overall sensitivity of 99.07%, and specificity of 100%. Sensitivity at parasite densities < 200, 200–2000, and > 2000 parasites/μL was 87.5, 98.7, and 100%, respectively.

Conclusions

These results suggest that HRP 2 exon II contains immunogenic sites similar to those of the native antigen and can be used for the development of mAbs suitable for malaria diagnosis in endemic communities.

【 授权许可】

   
2015 Kang et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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