Genome-wide methylation profiling of the bronchial mucosa of asthmatics: relationship to atopy

BMC Medical Genetics

Choon-Sik Park¹  Hyoung Doo Shin²  Hyun Sub Cheong³  Jong-Sook Park¹  Tae-Hoon Kim¹  Sung-Woo Park¹  Yoon-Jeong Kim¹ 

[1] Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, 1174, Jung Dong, Wonmi-Gu, Bucheon, Gyeonggi Do 420-021, Korea;Department of Life Science, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, Korea;Department of Genetic Epidemiology, SNP Genetics, Inc., Room 1407, 14th floor, B-dong, WooLim Lion’s Valley, 371-28, Gasan-dong, Geumcheon-Gu, Seoul 153-803, Korea

关键词: Environmental factor;    Epigenetics;    DNA methylation;    Atopy;    Bronchial mucosa;    Asthma;   

Others  :  1177714
DOI  :  10.1186/1471-2350-14-39

 received in 2012-08-29, accepted in 2013-03-14,  发布年份 2013

【 摘 要 】

Background

Asthma is a common respiratory disease that is characterized by bronchial hyperresponsiveness and airway obstruction due to chronic airway inflammation. Atopic asthma is a typical IgE-mediated disease in which the enhanced production of IgE is driven by the activation of Th2 cells, which release a distinct pattern of cytokines, including interleukin 4 (IL4) and IL3, in response to specific antigen presentation. To evaluate the methylation status of the whole genomes of bronchial mucosa tissues from subjects who lacked or had sensitization to Dermatophagoides farina (Df) and Dermatophagoides pteronyssinus (Dp).

Methods

The genome-wide DNA methylation levels in the bronchial mucosa tissues of atopic asthmatics (N = 10), non-atopic asthmatics (N = 7), and normal controls (N = 7) were examined using microarrays.

Results

In the bronchial mucosa of atopic asthmatics, hypermethylation was detected at 6 loci in 6 genes, while hypomethylation was detected at 49 loci in 48 genes compared to those of non-atopic asthmatics. Genes that were assigned the ontologies of multicellular organismal process, response to organic substance, hormone metabolic process, and growth factor receptor binding were hypomethylated. The methylation levels in the mucosa of asthmatics and normal controls were similar.

Conclusions

The bronchial mucosa of asthmatics who are atopic to Df or Dp have characteristic methylation patterns for 52 genes. The genes and pathways identified in the present study may be associated with the presence of atopy in asthmatics and therefore represent attractive targets for future research.

【 授权许可】

   
2013 Kim et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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