Differential marker expression by cultures rich in mesenchymal stem cells

BMC Cell Biology

Chaker Adra¹  Imaduddin Kanaan³  Ameera Gaafar²  Hind Al-Humaidan⁴  Hazem Ghebeh²  Ghida Majed Sleiman²  Zakia Shinwari²  Amer Al-Mazrou²  Manogaran S Pulicat²  Christian Benedict Pradez²  Monther Al-Alwan²  Ayodele Alaiya²  Andrew Wetzig² 

[1] Transplantation Research Centre (TRC), Brigham & Women’s Hospital and Children’s Hospital Boston, Harvard Medical School, Boston, MA, USA;Stem Cell & Tissue Re-engineering Program, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Kingdom of Saudi Arabia;Department of Neurosciences, King Faisal Specialist Hospital & Research Centre, Riyadh, Kingdom of Saudi Arabia;Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital & Research Centre, Riyadh, Kingdom of Saudi Arabia

关键词: Fibroblasts and olfactory;    Breast adipose stem cell;    Bone marrow mesenchymal stem cell;    Cell surface markers;    Mesenchymal stem cell;   

Others  :  855035
DOI  :  10.1186/1471-2121-14-54

 received in 2013-08-28, accepted in 2013-11-25,  发布年份 2013

【 摘 要 】

Background

Mesenchymal stem cells have properties that make them amenable to therapeutic use. However, the acceptance of mesenchymal stem cells in clinical practice requires standardized techniques for their specific isolation. To date, there are no conclusive marker (s) for the exclusive isolation of mesenchymal stem cells. Our aim was to identify markers differentially expressed between mesenchymal stem cell and non-stem cell mesenchymal cell cultures. We compared and contrasted the phenotype of tissue cultures in which mesenchymal stem cells are rich and rare. By initially assessing mesenchymal stem cell differentiation, we established that bone marrow and breast adipose cultures are rich in mesenchymal stem cells while, in our hands, foreskin fibroblast and olfactory tissue cultures contain rare mesenchymal stem cells. In particular, olfactory tissue cells represent non-stem cell mesenchymal cells. Subsequently, the phenotype of the tissue cultures were thoroughly assessed using immuno-fluorescence, flow-cytometry, proteomics, antibody arrays and qPCR.

Results

Our analysis revealed that all tissue cultures, regardless of differentiation potential, demonstrated remarkably similar phenotypes. Importantly, it was also observed that common mesenchymal stem cell markers, and fibroblast-associated markers, do not discriminate between mesenchymal stem cell and non-stem cell mesenchymal cell cultures. Examination and comparison of the phenotypes of mesenchymal stem cell and non-stem cell mesenchymal cell cultures revealed three differentially expressed markers – CD24, CD108 and CD40.

Conclusion

We indicate the importance of establishing differential marker expression between mesenchymal stem cells and non-stem cell mesenchymal cells in order to determine stem cell specific markers.

【 授权许可】

   
2013 Wetzig et al.; licensee BioMed Central Ltd.

【 图 表 】

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