Comparative genomic analysis of clinical and environmental strains provides insight into the pathogenicity and evolution of Vibrio parahaemolyticus

BMC Genomics

Hoi Shan Kwan²  Kai Man Kam¹  Patrick Tik Wan Law²  Man Kit Cheung²  Wenyan Nong²  Hin-chung Wong³  Lei Li⁴ 

[1]Stanley Ho Centre for Emerging Infectious Diseases, JC School of Public Health, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, People’s Republic of China

[2]School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, People’s Republic of China

[3]Department of Microbiology, Soochow University, Taipei 111, Taiwan

[4]Present address: Institute for Molecular Infection Biology, University of Würzburg, Würzburg, Germany

关键词: Environment;    Clinical;    Comparative genomics;    Vibrio parahaemolyticus;   

Others  :  1125717
DOI  :  10.1186/1471-2164-15-1135

 received in 2014-09-05, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

Vibrio parahaemolyticus is a Gram-negative halophilic bacterium. Infections with the bacterium could become systemic and can be life-threatening to immunocompromised individuals. Genome sequences of a few clinical isolates of V. parahaemolyticus are currently available, but the genome dynamics across the species and virulence potential of environmental strains on a genome-scale have not been described before.

Results

Here we present genome sequences of four V. parahaemolyticus clinical strains from stool samples of patients and five environmental strains in Hong Kong. Phylogenomics analysis based on single nucleotide polymorphisms revealed a clear distinction between the clinical and environmental isolates. A new gene cluster belonging to the biofilm associated proteins of V. parahaemolyticus was found in clincial strains. In addition, a novel small genomic island frequently found among clinical isolates was reported. A few environmental strains were found harboring virulence genes and prophage elements, indicating their virulence potential. A unique biphenyl degradation pathway was also reported. A database for V. parahaemolyticus (http://kwanlab.bio.cuhk.edu.hk/vp webcite) was constructed here as a platform to access and analyze genome sequences and annotations of the bacterium.

Conclusions

We have performed a comparative genomics analysis of clinical and environmental strains of V. parahaemolyticus. Our analyses could facilitate understanding of the phylogenetic diversity and niche adaptation of this bacterium.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central.

【 图 表 】

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