Assessment of contamination using an ATP bioluminescence assay on doorknobs in a university-affiliated hospital in Japan

BMC Research Notes

Takashi Takahashi³  Noritada Kobayashi²  Makoto Ikenaga²  Satsuki Hata²  Norio Yokota²  Tomohiro Fujita¹  Shinta Koike¹  Yoneji Hirose¹  Naoko Kajigaya² 

[1] Department of Clinical Laboratory, Kitasato University Medical Center, 6-100 Arai, Kitamoto, Saitama 364-8501, Japan;Department of Infection Control and Prevention, Kitasato University Medical Center, 6-100 Arai, Kitamoto, Saitama 364-8501, Japan;Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan

关键词: University hospital;    Stamp culture;    ATP bioluminescence assay;    Contamination;    Doorknob;   

Others  :  1230884
DOI  :  10.1186/s13104-015-1305-7

 received in 2015-03-19, accepted in 2015-07-28,  发布年份 2015

【 摘 要 】

Background

Doorknobs are inevitable points of hand contact. We monitored doorknob contamination in a university hospital using an ATP bioluminescence assay and stamp agar method. We selected grip-, lever-, push-, insert-, and two-pull-type doorknobs in staff lavatories and break rooms, a linen closet, dirty utility rooms, a newborn care unit, clinical lavatories and examination rooms, dressing rooms for radiological tests, and lavatories for health examination, as monitoring points in wards and clinics. Sequential monitoring with an ATP assay (six times) and culture (once) were performed at the same time of day in autumn, winter, and summer. We provided contamination data to appropriate healthcare providers and housekeepers, and queried the staff regarding decontamination of doorknobs.

Results

When comparing ATP values on the same type of doorknobs, significant differences in contamination were demonstrated among several clinical rooms and several rooms in wards during all three seasons. No correlation was observed between ATP values on clinical-examination-room doorknobs and outpatient numbers, or between ATP values at any monitoring point and microbial colony-forming units. ATP values on clinical-examination-room doorknobs were reduced after cleaning according to instructions.

Conclusions

ATP assay is useful for measuring baseline doorknob contamination in clinical rooms. Our findings confirm the need to improve routine decontamination in clinical departments. We need to analyze further the relationship between hospital-acquired infections and doorknob contamination, as assessed by ATP assay in clinics.

【 授权许可】

   
2015 Kajigaya et al.

【 图 表 】

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