Association of the UCP-1 single nucleotide polymorphism A-3826G with the dampness-phlegm pattern among Korean stroke patients

BMC Complementary and Alternative Medicine

Myeong Soo Lee¹  Min Ho Cha¹  Tae-Woong Moon¹  Mi Mi Ko¹  Ji Hye Lim¹ 

[1] Medical Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Republic of Korea

关键词: Triglyceride;    Ala64Thr;    A-1766G;    A-3826G;    Dampness-phlegm;    Polymorphism;    UCP-1;   

Others  :  1231943
DOI  :  10.1186/1472-6882-12-180

 received in 2012-06-13, accepted in 2012-09-26,  发布年份 2012

【 摘 要 】

Background

Patients with stroke have various syndromes and symptoms. Through pattern identification (PI), traditional Korean medicine (TKM) classifies the several syndromes and symptoms of stroke patients into five categories: Fire-heat (FH), Dampness-phlegm (DP), Yin-deficiency (YD), Qi-deficiency (QD) and Blood-stasis (BS).DP has been associated with obesity and hyperlipidemia. Uncoupling protein-1 (UCP-1), which plays a major rolein thermogenesis and energy expenditure can increase the risk of obesity and can be related metabolic disorders. In this study, we elucidated the association of three polymorphisms located in the UCP-1 promoter and coding region with DP among Korean stroke patients.

Methods

1,593 patients with cerebral infarction (583/DP, 1,010/non-DP) and 587 normal subjects were enrolled. The genotypes A-3826G, G-1766A and Ala64Thr (G+1068A) for each subject were determined by polymerase chain reaction with TaqMan probes and five percent of subjects were re-genotyped by sequencing method to confirm the accuracy of genotyping. The results were analyzed using a multiple logistic regression model to evaluate the genetic associations: the UCP-1polymorphisms of normal versus those of DP subjects and those of normal versus those of non-DP subjects.

Results

A significantly higher percentage of subjects in the DP group possessed the A-3826G G allele than theA allele (OR=1.508, p=0.006). Furthermore, the number of subjects with the GG type of A-1766G was significantly lower in the non-DP group than the normal group in the recessive model (OR=0.606, p=0.042). In addition, an analysis of the relationship among 2 SNPs of UCP-1 and lipid serum concentration showed that the serum levelof HDL cholesterol was significantly higher in subjects with the A-3826G G allele in the normal group (p=0.032).Serum triglyceride and HDL cholesterol were also associated with the A-1766G variant in the recessive model (p=0.002, p=0.046).

Conclusions

These results suggest that that the A-3826G and A-1766G UCP-1 polymorphisms, which are relatedto obesity, might be candidate genetic markers for the DP pattern in the TKM diagnosis system.

【 授权许可】

   
2012 Lim et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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