Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination

BMC Infectious Diseases

Katie L Flanagan¹  Sarah Crozier³  Marianne van der Sande⁴  Hilton Whittle¹  Ebrima S Touray¹  Olubukola Ojuola²  Melba S Palmero¹  Pauline Waight⁵  David JC Miles⁶  Brigitte Walther¹ 

[1]MRC Laboratories Gambia, PO Box 273, Banjul, Gambia

[2]Department of Pediatrics, Bronx Lebanon Hospital Center, 1650 Selwyn Avenue, Bronx, New York, USA

[3]MRC Epidemiology Resource Centre, University of Southampton, Southampton General Hospital, Southampton, UK

[4]Julius Centre, University Medical Centre Utrecht, Utrecht, The Netherlands

[5]Immunisation Department, Health Protection Agency Centre for Infections, London, UK

[6]Malawi-Liverpool-Wellcome Trust Clinical Research Programme, PO Box 30096, Chichiri, Blantyre 3, Malawi

Others  :  1175484
DOI  :  10.1186/1471-2334-12-6

 received in 2011-11-07, accepted in 2012-01-14,  发布年份 2012

【 摘 要 】

Background

Placental malaria (PM) is associated with prenatal malaise, but many PM+ infants are born without symptoms. As malaria has powerful immunomodulatory effects, we tested the hypothesis that PM predicts reduced T-cell responses to vaccine challenge.

Methods

We recruited healthy PM+ and PM- infants at birth. At six and 12 months, we stimulated PBMCs with tuberculin purified protein derivative (PPD) and compared expression of CD154, IL-2 and IFNγ by CD4 T-cells to a negative control using flow cytometry.

We measured the length, weight and head circumference at birth and 12 months.

Results

IL-2 and CD154 expression were low in both groups at both timepoints, without discernable differences. Expression of IFNγ was similarly low at 6 months but by 12 months, the median response was higher in PM- than PM + infants (p = 0.026). The PM+ infants also had a lower weight (p = 0.032) and head circumference (p = 0.041) at 12 months, indicating lower growth rates.

At birth, the size and weight of the PM+ and PM- infants were equivalent. By 12 months, the PM+ infants had a lower weight and head circumference than the PM- infants.

Conclusions

Placental malaria was associated with reduced immune responses 12 months after immune challenge in infants apparently healthy at birth.

【 授权许可】

   
2012 Walther et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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