Possible modulation of FAS and PTP-1B signaling in ameliorative potential of Bombax ceiba against high fat diet induced obesity

BMC Complementary and Alternative Medicine

Pyare Lal Sharma¹  Yamini Chauhan²  Rohit Goyal²  Paras Gupta¹ 

[1] Department of Pharmacology, ISF College of Pharmacy, Moga 142 001, India;School of Pharmaceutical Sciences, Shoolini University, Solan, HP 173212, India

关键词: Fatty acid synthase;    Protein tyrosine phosphatase-1B;    Free fatty acid;    Bombax ceiba;    Obesity;   

Others  :  1220879
DOI  :  10.1186/1472-6882-13-281

 received in 2012-12-31, accepted in 2013-09-25,  发布年份 2013

【 摘 要 】

Background

Bombax ceiba Linn., commonly called as Semal, is used in various gastro-intestinal disturbances. It contains Lupeol which inhibits PTP-1B, adipogenesis, TG synthesis and accumulation of lipids in adipocytes and adipokines whereas the flavonoids isolated from B. ceiba has FAS inhibitory activity. The present study was aimed to investigate ameliorative potential of Bombax ceiba to experimental obesity in Wistar rats, and its possible mechanism of action.

Methods

Male Wistar albino rats weighing 180-220 g were employed in present study. Experimental obesity was induced by feeding high fat diet for 10 weeks. Methanolic extract of B. ceiba extract 100, 200 and 400 mg/kg and Gemfibrozil 50 mg/kg as standard drug were given orally from 7^th to 10^th week.

Results

Induction with HFD for 10 weeks caused significant (p < 0.05) increase in % body wt, BMI, LEE indices; serum glucose, triglyceride, LDL, VLDL, cholesterol, free fatty acid, ALT, AST; tissue TBARS, nitrate/nitrite levels; different fat pads and relative liver weight; and significant decrease in food intake (g and kcal), serum HDL and tissue glutathione levels in HFD control rats. Treatment with B. ceiba extract and Gemfibrozil significantly attenuated these HFD induced changes, as compared to HFD control. The effect of B. ceiba 200 and 400 mg/kg was more pronounced in comparison to Gemfibrozil.

Conclusion

On the basis of results obtained, it may be concluded that the methanolic extract of stem bark of Bombax ceiba has significant ameliorative potential against HFD induced obesity in rats, possibly through modulation of FAS and PTP-1B signaling due to the presence of flavonoids and lupeol.

【 授权许可】

   
2013 Gupta et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

【 参考文献 】

• [1]Grill H, Ginsberg A, Seeley R, Kaplan J: Brainstem application of melanocortin receptor ligands produces long-lasting effects on feeding and body weight. J Neurosci 1998, 18:10128-10135.
• [2]Haslam DW, James WP: Obesity. Lancet 2005, 366:1197-1209.
• [3]Wilding JP: Neuropeptides and appetite control. Diabet Med 2001, 19:619-627.
• [4]Zammit VA, Waterman IJ, Topping D, McKay G: Insulin stimulation of hepatic triacylglycerols secretion and the etiology of insulin resistance. J Nutr 2001, 131:2074-2077.
• [5]Klaman LD, Boss O, Peroni OD, Kim JK, Martino JL, Zabolotny JM, Moghal N, Lubkin M, Kim YB, Sharpe AH: Increased energy expenditure, decreased adiposity, and tissue-specic insulin sensitivity in protein-tyrosine phosphatase 1B-decient mice. Mol Cell Biol 2000, 20:5479-5489.
• [6]Sharma H, Chandola HM: Ayurvedic concept of obesity, metabolic syndrome, and diabetes mellitus. J Altern Complem Med 2011, 17:549-552.
• [7]Verma V, Jalalpure SS, Sahu A, Bhardwaj LK, Prakesh Y: Bombax ceiba Linn: pharmacognostical, phytochemistry, ethnobotany, and pharmacology studies. Int Pharm Sci 2011, 1:62-68.
• [8]Young-jae Y, Nguyen-Hai N, Young K, Byung-Zun A: Antiangiogenic activity of lupeol from Bombax ceiba stems bark. Phyto Reas 2003, 17(4):341-344.
• [9]Saleem R, Ahmad M, Hussain SA, Qazi AM, Ahmad SI: Hypotensive, hypoglycemic and toxicology studies on the flavonol C-glycoside shamimin from Bombax ceiba. Planta Med 1999, 65:331-334.
• [10]Dar A, Faizi S, Naqvi S, Roome T, Zikrur-Rehman S, Ali M, Firdous S, Moin ST: Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biol Pharm Bull 2005, 28:596-600.
• [11]Singh V, Pandey RP: Ethanobotany of Rajasthan India, scientific publishers. Jodhpur 1998, 63:58-59.
• [12]Karnick CR: Ethnobotanical records of drug plants described in valmiki Ramayana and their uses in the ayurvedic system of medicine. Quart J Crude Drug Res 1975, 13:143-154.
• [13]Jain V, Verma SK, Katewa SS, Anandjiwala S, Sing B: Free radical scavenging property of Bombax ceiba Linn. root. Res J Med Plant 2011, 5:462-470.
• [14]Kumar S: Evaluation of RBC membrane stabilization and antioxidant activity of Bombax ceiba in an in vitro method. Int J Pharma and Bio Sci 2011, 2:1.
• [15]Chen J, Zhuang D, Cai W, Xu L, Li E, Wu Y, Sugiyama K: Inhibitory effects of four plants flavonoids extracts on fatty acid synthase. J Environ Sci 2009, 21:S131-S134.
• [16]Hata K, Hori K, Murata J, Takahashi S: Remodeling of actin cytoskeleton in lupeol induced B16 2F2 cell differentiation. J Biochem 2005, 138(4):467-472.
• [17]Harborne JB: Phytochemical methods. London: Chapman and Hall; 1998:1-254. 3
• [18]Botham PA: Acute systemic toxicity prospects for tiered testing strategies. Toxicol in Vitro 2004, 18:227-230.
• [19]Shrinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P: Combination of high-fat-diet and low-dose steptozotocin-trated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res 2005, 52:313-320.
• [20]Novellie LB, Dinij YS, Galhardi CM, Ebaid GMX, Rodrigues HG, Main F, Fernandes AAH, Cicogna AC, Novellifilho JLVB: Anthropometrical parameters and markers of obesity in rats. Lab Animals 2007, 41:111-119.
• [21]Bernardis LL: Prediction of carcass fat, waterand lean body mass from Lee’s nutritive ratio in rats with hypothalamic obesity. Experientia 1970, 26:789-790.
• [22]Ainsline DA, Proietto J, Fam BC, Thormburn AW: Short-term, high-fat diet low circulating leptin concentration in rats. Am J Clin Nutr 2000, 71:438-442.
• [23]Trinder K, Hiraga Y, Nakamura N, Kitajo A, Linuma F: Determination of glucose in blood using glucose oxidase-peroxidien. Chem Phem Bulletin 1969, 27:568-570.
• [24]Allain CC, Poon LS, Chan CS, Richmond W, Fu PC: Enzymatic determination of total serum cholesterol. Clin Chem 1974, 20:470-475.
• [25]Friedewal WT, Levy RI, Fredrickson DS: Estimation of the concentration of low-density lipoprotein in cholesterol in plasma, without use of the preparative ultracentrifuge. Cin Chem 1972, 18:499-502.
• [26]Werner M, Gabrielson DG, Eastman J: Ultramicro determination of serum triglyceride by bioluminescent assay. Clin Chem 1981, 27:268-271.
• [27]Clin J: IFCC method for the measurement of catalytic concentration of enzyme. Chem Clin Biochem 1986, 24:497.
• [28]Christie WW: Preparation of ester derivatives of fatty acids for chromatographic analysis. In Advances in Lipid Methodology. Edited by Christie WW. Dundee: Oily Press; 1993:69-111. 2
• [29]Ohkawa Η, Ohishi Ν, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979, 95:351.
• [30]Ellman GL: Tissue sulphydryl groups. Arch Biochem Biophys 1959, 82:70-77.
• [31]Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR: Analysis of nitrate, nitrite, and nitrate in biological samples. Anal Biochem 1982, 126:131-138.
• [32]Buettner R, Parhofer KG, Woenckhaus M, Wrede CE, Kunz-Schughart LA, Scholmerich J, Bollheimer LC: Defining high fat diet rat models: metabolic and molecular effects of different fat types. J Mol Endocrinol 2006, 36:485-501.
• [33]Chen HC, Farese RVJ: Determination of adipocyte size by computer image analysis. J Lipid Res 2002, 43:986-989.
• [34]Toruner F, Akbay E, Cakir N, Sancak B, Elbeg S, Taneri F, Aktürk M, Karakoç A, Ayva ZG, Arslan M: Effects of PPAR gamma and PPAR alpha agonists on serum leptin levels in diet-induced obese rats. Horm Metab Res 2004, 36:226-230.
• [35]Pang J, Choi Y, Park T: Ilex paraguariensis exart ameliorate obesity induced by high fat diet: potential rale of AMPK in the visceral adipose tissue. Arch Biochem Biophys 2008, 476(2):178-185.
• [36]Woods SC, Seeley RJ, Rushing PA, D’Alessio D, Tso PA: Controlled high fat diet induced an obese syndrome in rats. J Nutr 2003, 133:1081-1087.
• [37]Storlien LH, Jenkins AB, Chisholm DJ, Pascoe WS, Khouri S, Kraegen EW: Influence of dietary fat composition on development of insulin resistance in rats. Relationship to muscle triglyceride and n-3 fatty acids in muscle phospholipids. Diabetes 1991, 40(2):280-289.
• [38]Gil-Campos M, Canete R, Gil A: Hormones regulating lipid metabolism and plasma lipids in childhood obesity. Int J Obes Relat Metab Disord 2003, 3:S75-S80.
• [39]Wanless IR, Lentz JS: Fatty liver hepatitis (steatohepatitis) and obesity: an autopsy study with analysis of risk factors. Hepatology 1990, 12:1106-1110.
• [40]Unger RH: The physiology of cellular liporegulation. Annu Rev Physiol 2003, 65:333-347.
• [41]Hyashi T, Hirshman MF, Kurth EJ, Winder WW, Goodyear LJ: Evidence for 5′AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport. Diabetes 1998, 47:1369-1373.
• [42]Day CP, James OF: Steatohepatitis: a tale of two hits. Gastroenterology 1998, 114:842-845.