Chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the Mediterranean cypress (Cupressus sempervirens L.)

BMC Complementary and Alternative Medicine

Abdulrhman R Albalawi³  Sherif M Hassan⁴  Mohammed E Adam²  Samy A Selim¹ 

[1] Botany Department, Faculty of Science, Suez Canal University, P.O. 41522, Ismailia, Egypt;West Kordufan University (WKU), Nuhud, Sudan;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, P.O. 2014, Sakaka, Saudi Arabia;Botany Department, Faculty of Science, Beni Suef University, Beni Suef, Egypt

关键词: Antibiofilm activity;    Antimicrobial activity;    GC-MS;    Essential oil;    Methanol extract;    Mediterranean Cupressus sempervirens L;   

Others  :  1220114
DOI  :  10.1186/1472-6882-14-179

 received in 2013-10-12, accepted in 2014-05-28,  发布年份 2014

【 摘 要 】

Background

Cupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive.

Methods

The present study was conducted to evaluate the in vitro antimicrobial, antibiofilm and determination chemical contents of the essential oil (Eo) and methanol extract from Mediterranean C. sempervirens L. The chemical composition of a hydrodistilled Eo of C. sempervirens was analyzed by a GC and GC/MS system.

Results

A total of 20 constituents representing 98.1% of the oil were identified: α-pinene (48.6%), δ-3-carene (22.1%), limonene (4.6%) and α-terpinolene (4.5%) were the main components comprising 79.8% of the oil. The antimicrobial test results showed that the methanol extract of C. sempervirens strongly inhibited the growth of the test bacteria studied, except for yeast species while the Eo had moderate antibacterial, but no anti-candida activity. Klebsiella pneumoniae was proven to be the most susceptible against methanol extract. The exposure time of Eo and methanol extract for complete inhibition of cell viability of K. pneumoniae was found to be 250 μg at 30 min and 500 μg at 120 min, respectively. The antibiofilm potential of the samples was evaluated using methods of PVC microtiter and eradication on biomaterial. Visual results showed visible biofilm eradication from the surface of intravenous infusion tube at 500 μg of Eo and methanol extract.

Conclusions

The results presented here may suggest that the Eo and extracts of C. sempervirens possess antimicrobial and antibiofilm properties, and therefore, can be used as natural preservative ingredients in food and/or pharmaceuticals.

【 授权许可】

   
2014 Selim et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Rawat P, Khan MF, Kumar M, Tamarkar AK, Srivastava AK, Arya KR, Maurya R: Constituents from fruits of Cupressus sempervirens. Fitoterapia 2010, 81:162-166.
• [2]Mascolo N, Autore G, Capasso F, Menghini A, Fasulo MP: Biological screening of Italian medicinal plants for anti-inflammatory activity. Phytother Res 1987, 1:28-31.
• [3]Usher GA: Dictionary of plants used by Man. London: Constable and Company; 1974.
• [4]Nehdi IA: Cupressus sempervirens var. Horizentalis seed oil: chemical composition, physicochemical characteristics, and utilizations. Ind Crop Prod 2013, 41:381-385.
• [5]Pierre-Leandri C, Fernandez X, Lizzani-Cuvelier L, Loiseau AM, Fellous R, Garnero J, Andréoli C: Chemical composition of cypress essential oils: volatile constituents of leaf from seven cultivated Cupressus species. J Essent Oil Res 2003, 15:242-247.
• [6]Chanegriha N, Baâliouamer A, Meklati BY, Favre-Bonvin J, Alamercery S: Chemical composition of Algerian cypress essential oil. J Essent Oil Res 1993, 5:671-674.
• [7]Chanegriha N, Baâliouamer A, Meklati BY, Chretien JR: Keravis G: GC and GC/MS leaf oil analysis of four Algerian cypress species. J Essent Oil Res 1997, 9:555-559.
• [8]Tapondjou AL, Adler C, Fontem DA, Bouda H, Reichmuthm C: Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. J Stored Prod Res 2005, 41:91-102.
• [9]Mazari K, Bendimerad N, Bekhechi C, Fernandez X: Chemical composition and antimicrobial activity of essential oils isolated from Algerian Juniperus phoenicea L. and Cupressus sempervirens L. J Med Plants Res 2010, 4:959-964.
• [10]Emamia SA, Asilia J, Rahimizadehb M, Fazly-Bazzazc BS, Khayyat MH: Chemical and antimicrobial studies of Cupressus sempervirens L. and C. horizentalis Mill. essential oils. Iran J Pharm Sci 2006, 2:103-108.
• [11]Boukhris M, Regane G, Yangui T, Sayadi S, Bouaziz M: Chemical composition and biological potential of essential oil from Tunisian Cupressus sempervirens L. J Arid Land Stud 2013, 22–1:329-332.
• [12]Adams RP: Quadrupole mass spectra of compounds listed in order of their retention time on DB-5. Stream, IL, USA: Allured Publishing Corporation, Carol; 2001:456. [Identification of essential oils components by gas chromatography/quadrupole mass spectroscopy]
• [13]Selim SA, Abdel Aziz MH, Mashait MS, Warrad MF: Antibacterial activities, chemical constitutes and acute toxicity of Egyptian Origanum majorana L., Peganum harmala L. and Salvia officinalis L. essential oils. Afr J Pharm Pharmacol 2013, 7:725-735.
• [14]Selim SA, El Alfy S, Al-Ruwaili M, Abdo A, Al Jaouni S: Susceptibility of imipenem-resistant Pseudomonas aeruginosa to flavonoid glycosides of date palm (Phoenix dactylifera L.) tamar growing in Al Madinah, Saudi Arabia. Afr J Biotechnol 2012, 11:416-422.
• [15]Selim SA: Chemical composition, antioxidant and antimicrobial activity of the essential oil and methanol of the Egyptian lemongrass Cymbopogon proximus Stapf. Grasas y Aceites 2011, 62:55-61.
• [16]Selim SA: Antimicrobial, antiplasmid and cytotoxicity potentials of marine algae Halimeda opuntia and Sarconema filiforme collected from Red Sea Coast. World Acad Sci Eng Technol 2012, 61:1154-1159.
• [17]Djordjevic D, Wiedmann M: Mclands borough LA: microtitre plate assay for assessment of Listeria monocytogenes biofilm formation. Appl Environ Microbiol 2002, 68:2950-2958.
• [18]Xiao J, Zuo Y, Liu Y, Li J, Hao Y, Zhou X: Effects of Nidus Vespae extract and chemical fractions on adherence and biofilm formation of Streptococcus mutans. Arch Oral Biol 2007, 52:869-875.
• [19]Burt S: Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol 2004, 94:223-253.
• [20]Delamare APL, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S: Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem 2007, 100:603-608.
• [21]Selim S, Hassan S, Al Soumaa K, EL Anzy S: Prevalence, antibiotic resistance and in vitro activity of yogurt against some gram negative pathogenic bacteria isolated from Arar Hospital, KSA. Life Sci J 2013, 10:1450-1456.
• [22]Demirci B, Kosar M, Demirci F, Dinc M, Baser KHC: Antimicrobial and antioxidant activities of the essential oil of Chaerophyllum libanoticum Boiss. et Kotschy. Food Chem 2007, 105:1512-1517.
• [23]Yang JK, Choi MS, Seo WT, Rinker DL, Han SW, Cheong GW: Chemical composition and antimicrobial activity of Chamaecyparis obtusa leaf essential oil. Fitoterapia 2007, 78:149-152.
• [24]Dorman HJD, Deansm SG: Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 2000, 88:308-316.
• [25]Cerca N, Martins S, Pier GB, Oliveira R, Azeredo J: The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm. Res Microbiol 2005, 156:650-655.
• [26]Kumar CG, Anand SK: Significance of microbial biofilms in food industry: a review. Int J Food Microbiol 1998, 42:9-27.
• [27]Klueh I, Wagner V, Kelly S, Johnson A, Bryers JD: Efficacy of silver- coated fabric to prevent bacterial colonization and subsequent device-based biofilm formation. J Biomed Mater Res 2000, 53:621-631.
• [28]Hashimoto H: Evaluation of the anti-biofilm effect of a new antibacterial silver citrate/lecithin coating in an in vitro experimental system using a modified robins device. J Jpn Assoc Infect Dis 2001, 75:678-685.
• [29]Meadows PS: The attachment of bacteria to solid surfaces. Arch Microbiol 1971, 75:374-381.
• [30]Ofek I, Hasty DL, Sharon N: Anti-adhesion therapy of bacterial diseases: prospects and problems. FEMS Immunol Med Microbiol 2003, 38:181-191.