Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats

BMC Complementary and Alternative Medicine

Chinaka Onyebuchi Nwaehujor⁴  Madubuike Umunna Anyanwu²  Chijioke Clement Muogbo³  Rita Ijoema Udegbunam¹  Sunday Ositadinma Udegbunam¹ 

[1] Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria;Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria;Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria;Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, Calabar, Nigeria

关键词: Ointments;    Antibacterial;    Wounds;    Pupalia lappacea;   

Others  :  1159793
DOI  :  10.1186/1472-6882-14-157

 received in 2013-11-25, accepted in 2014-04-29,  发布年份 2014

【 摘 要 】

Background

Wound healing is a natural process that enables tissue repair after an injury. To shorten its duration and minimize associated complications, wounds are treated with medications. Currently there is a growing interest in the use of alternative wound dressing agents such as plant extracts. One plant used traditionally in wound treatment is Pupalia lappacea. In view of its use in wound care, we investigated the wound healing activities of 80% methanolic leave extract of Pupalia lappacea using excision, incision and dead space wound models. Also its effects on three common wound contaminants were investigated.

Methods

Excision wounds were created, contaminated with microbes and treated with ointments (10% and 20% w/w) prepared from Pupalia lappacea. Incision and dead space wounds were also created in rats which were subsequently dosed orally with the extract. The wound healing activities of Pupalia lappacea ointment on excision wound was assessed by rates of wound contraction and epithelialization as well as its antibacterial effects. The effects of Pupalia lappacea on incision and dead-space wounds were determined by the wound breaking strengths and weights of the granuloma tissues formed, respectively.

Results

Pupalia. lappacea ointments significantly (p < 0.05) accelerated wound healing with 20% ointment having the highest percentage wound contraction and rate of epithelialization. At 4, 7 and 14 days post treatment, mean total viable bacterial count of excision wounds of the extract treated groups were significantly (p < 0.05) lower compared against the control. Wound breaking strengths and weights of granuloma tissues formed in the extract treated groups were significantly (p < 0.05) higher than those of the control group. The minimum inhibitory concentration values obtained for the Pupalia lappacea extract against Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis were 9 mg/ml, 4 mg/ml and 3 mg/ml, respectively, while the corresponding minimum bactericidal concentrations were 10 mg/ml, 8 mg/ml and 7 mg/ml.

Conclusion

The results obtained showed that Pupalia. lappacea has good wound healing and antibacterial activities. These findings validate the use of this plant in traditional medicine for treatment of wounds.

【 授权许可】

   
2014 Udegbunam et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Agyare C, Dwobeng AS, Agyepong N, Boakye YD, Mensah KB, Ayande PG, Adarkwa-Yiadom M: Antimicrobial, antioxidant, and wound healing properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC. Adv Pharmacol Sci 2013. http://dx.doi.org/10.1155/2013/692613 webcite
• [2]Raina R, Prawezi S, Verma PK, Pankaj NK: Medicinal plants and their role in wound healing. Vet Scand 2008, 3:1-7.
• [3]Waldron DR, Trevor P: Management of superficial skin wounds. In Textbook of small animal surgery volume 1. Edited by Slater D. Philadelphia: W. B. Saunders Company; 1993:269.
• [4]Nguyen DT, Orgill DP, Murphy GF: The pathophysiologic basis for wound healing and cutaneous regeneration. Cambridge/Boca Raton: Wood Head Publishing (UK/Europe) and CRC Press (US); 2009:25-57. [Biomaterials for treating skin loss]
• [5]Pandith H, Zhang X, Liggett J, Min K-Y, Gritsanapan W, Baek SJ: Hemostatic and wound healing properties of Chromolaena odorata leaf extract. ISRN Dermatol 2013. http://dx.doi.org/10.1155/2013/168269 webcite
• [6]Vezza R, Mezzasoma AM, Venditti G, Gresele P: Prostaglandin endoperoxides and thromboxane A2 activate the same receptor isoforms in human platelets. Thromb Haemost 2002, 87(1):114-121.
• [7]Aso Y: Plasminogen activator inhibitor (PAI)-1 in vascular inflammation and thrombosis. Front Biosci 2007, 12(8):2957-2966.
• [8]Martin P, Leibovich SJ: Inflammatory cells during wound repair: the good, the bad and the ugly. Trends Cell Biol 2005, 15(11):599-607.
• [9]Fialkow L, Wang Y, Downey GP: Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function. Free Radical Biol Med 2007, 42(2):153-164.
• [10]Wagener FADTG, van Beurden HE, von den Hoff GW, Adema GJ, Figdor CG: The heme-heme oxygenase system: a molecular switch in wound healing. Blood 2003, 102(2):521-528.
• [11]Stamenkovic I: Extracellular matrix remodelling: the role of matrix metalloproteinases. J Pathol 2003, 200(4):448-464.
• [12]Myers KA, Marshal RD, Friedin J: Principles of Pathology in Surgery 1st edition. London: Blackwell Scientific Publications; 1980:58-82.
• [13]Ravi A, Alvala M, Sama V, Kalle AM, Irlapati VK, Reddy BM: Anticancer activity of Pupalia lappacea on chronic myeloid leukemia K562 cells. DARU J Pharm Sci 2012, 20:86. BioMed Central Full Text
• [14]Udegbunam SO, Nnaji TO, Udegbunam RI, Okafor JC, Agbo I: Evaluation of herbal ointment formulation of Milicia excelsa (Welw) C.C berg for wound healing. Afr J Biotechnol 2013, 12(21):3351-3359.
• [15]Burkill HM: The useful plants of west tropical Africa. Kew Publishing; 1985:1:1.
• [16]Rao PP, Reddy RP: A note on folklore treatment of bone fracture from Ranga Reddy District, Andhra Pradesh. Ethnobotany 1999, 11:107-108.
• [17]Reddy SC, Reddy KN, Murthy EN, Raju US: Traditional medicinal plants used in Seshachalam Hills, Andhra Pradesh, India. J Med Plants Res 2009, 3:408-412.
• [18]Neeharika V, Fatima H, Reddy BM: Evaluation of antinociceptive and antipyretic effect of Pupalia lappacea Juss. Int Curr Pharm J 2013, 2(2):23-28.
• [19]Ndjonka D, Agyare C, Luersen K, Hensel A, Liebau E: In vitro antileishmanial activity of traditional medicinal plants from Cameroon and Ghana. Int J Pharm 2010, 6:863-871.
• [20]Bero J, Ganfon H, Jonville MC, Frederich M, Gbaguidi F, DeMol P, Moudachirou M, Quetin-Leclercq J: In vitro antiplasmodial activity of plants used in Benin in traditional medicine to treat malaria. J Ethnopharmacol 2009, 122:439-444.
• [21]Felix AA, Domingo O: Antioxidant activity and chemical constituents of P. lappacea (L) Juss. Res J Biol Sci 2008, 3:783-785.
• [22]Triratana T, Suwannuraks R, Naengchomnong W: Effect of eupatorium odoratum on blood coagulation. J Med Assoc Thai 1991, 74(5):283-287.
• [23]Gabay O, Sanchez C, Salvat C, Chevy F, Breton M, Nourissat G, Wolf C, Jacques C, Berenbaum F: Stigmasterol: a phytosterol with potential anti-osteoarthritic properties. Osteoarthritis Cartilage 2010, 18(1):106-116.
• [24]Lafont R, Dinan L: Practical uses for ecdysteroids in mammals including humans: an update. J Insect Sci 2003, 3(7):1-30.
• [25]McKeough MB, Spruance SL: Comparison of new treatment of Herpes labialis. Arch Dermatol 2001, 137:1153-1159.
• [26]Bobby MN, Wesely EG, Johnson MA: In vitro antibacterial activity of leaves extracts of Albizia lebbeck Benth against some selected pathogens. Asia-Pac Tropl Biomed 2012. doi:10.1016/S2221-1691(12)60324-4
• [27]Trease EC, Evans WC: Pharmacognosy. 12th edition. London: Bailliere and Tindall; 1983:115-625.
• [28]Okore VC, Ibezim EC, Adikwu MU, Attama AA, Esimone CO, Uzuegbu BD, Ofokansi KC: Laboratory techniques in Pharmaceutics and Pharmaceutical Microbiology. 2nd edition. El’Demark Publishers; 2004:1-20.
• [29]Baron JE, Finegold SM: Method for testing antimicrobial effectiveness. In Bailey Scott’s Diagnostic Microbiology. 8th edition. Edited by Mosby CV. Missouri: C. V. Mosby Company; 1990:21-23.
• [30]Morton JJ, Malone MM: Evaluation of vulnerary activity by open wound procedure in rats. J Trauma 1992, 20(4):323-324.
• [31]Ezike AC, Akah PA, Okoli CO, Udegbunam S, Okuma N, Okeke C, Iloani O: Medicinal plants used in wound care: a study of Prosopis africana (Fabaceae) stem bark. Indian Pharm Sci 2010, 72(3):334-338.
• [32]Nayak S, Nalabothu P, Sandiford S, Rhogdi V, Adogwa A: Evaluation of wound healing activity of Allamanda cathartica. L. and Laurus nobilis, L. extracts on rats. BMC Complement Altern Med 2006, 6:12. BioMed Central Full Text
• [33]Clinical and Laboratory Standards Institute (CLSI): Methods for dilution of antimicrobial susceptibility tests for bacteria that grow aerobically, approved standard. 7th edition. Wayne, Pa: Clinical and Laboratory Standards Institute document M7-A7; Clinical and Laboratory Standards Institute; 2006. 5–22
• [34]Rathi B, Patil PA, Baheti AM: Evaluation of aqueous extract and seeds of Moringa oleifera for wound healing in albino rats. J Nat Remedies 2004, 4(2):145-149.
• [35]Lee KH: Studies on mechanism of action of salicylates. retardation of wound healing by aspirin. J Pharm Sci 1986, 51(6):1042-1043.
• [36]Al-Henhena N, Mahmoood AA, Al-Magrami A, Nor SAB, Zahra AA, Summaya MD, Suzi MS, Salmah I: Histological study of wound healing potential by ethanol leaf extract of Strobilanthes crispus in rats. J Med Plants Res 2011, 5(16):3666-3669.
• [37]Mohan H: Inflammation and healing. In Textbook of Pathology. 5th edition. New Delhi: Jaypee Brothers; 2005:133-179.
• [38]Habibipour S, Oswald TM, Zhang F, Joshi P, Zhou XC, Dorsett MW, Lineaweaver WC: Effects of sodium diphenhydantion on skin wound healing in rats. Plast Reconstr Surg 2003, 2003(112):1620-1627.
• [39]Suntar IP, Koca U, Esra KA, Yilmazer D, Alper M: Assessment of wound healing activity of the aqueous extracts of Colutea cilicica fruits and leaves Boiss and Bal. Evid Based Altern Complement Med 2011, 2011:1-7.
• [40]Akkol ES, Koco U, Pesin L, Yilmazer D: Exploring the wound healing activity of Amebia densiflora. J Ethnopharmacol 2009, 124:137-141.
• [41]Suguna L, Singh S, SivaKumar P, Sampath P, Chandrakason G: Influence of terminalia chebula on dermal wound healing in rats. Phytother Res 2002, 16:227-231.
• [42]Buntrock P, Jentzsch KD, Heder G: Stimulation of wound healing using brain extract with fibroblast growth factor (FGF) activity II. histological and morphometric examination of cells and capillaries. Exp Pathol 1982, 21(1):62-67.
• [43]Cotran RS, Kumar V, Robbins SL, Schoem FJ: Inflammation and repair. In Robbins Pathologic Basis of Diseases. 5th edition. Pennsylvania: W. B. Saunders Company; 1994:51-92.
• [44]Trabucchi E, Preis BF, Barathi C, Montorisi W: Topical treatment of experimental skin lesions in rats macroscopic, microscopic and scanning electron microscopic evaluation of the healing process. Int J Tissue React 1986, 8:533-544.
• [45]Shukla A, Rasik AM, Dhawan RN: Asiaticoside-induced elevation of antioxidant levels in healing wounds. Phytother Res 1999, 13(1):50-54.
• [46]Cohen IK, Diegelmann RF, Lindblad WJ: Wound healing: Biochemical and Clinical Aspects. Philadelphia: W.B. Saunders Company; 1992:344-355.
• [47]Szabo S, Kusstatsher S, Sakoulas S, Sandor Z, Vincse A, Jadus M: Growth factors: new endogenous drug for ulcer healing. Scand J Gastroenterol 1995, 210:15-18.
• [48]Kumar MS, Sripriya R, Raghavan HV, Sehgal PK: Wound healing potential of Cassia fistula on infected albino rat model. J Surg Res 2006, 131:283-289.
• [49]Muhammad HS, Muhammad S: The use of Lawsonia inermis Linn. (henna) in the management of burn wound infections. Afr J Biotechnol 2005, 4:934-937.
• [50]Deshmukh PT, Fernandes J, Atul A, Toppo E: Wound healing activity of calotropis gigantean root bark in rats. J Ethnopharmacol 2009, 125:178-181.
• [51]Eming SA, Werner S, Bugnon P, Wicksiewe L, Utermohien O, Davidson JM, Krieg T, Roers A: Accelerated wound closure in mice deficient for interleukin-10. Am J Pathol 2007, 170(1):170-202.
• [52]Calabresse V, Scapagnini C, Catalano C, Dinotta F, Geraci D, Morganti P: Biochemical studies of a natural antioxidant isolated from Rosemary and its application in cosmetic dermatology. Int J Tissue React 2000, 22:5-13.
• [53]Aladedunye AF, Okorie DA: Antioxidant activity and chemical constituents of Pupalia Lappacea (L.) Juss. Res J Biol Sci 2008, 3(7):783-785.
• [54]Karou D, Savadogo A, Canini A, Yameogo S, Montesano C: Antibacterial activity from Sida acuta. Afr J Biotechnol 2006, 5(2):195-200.
• [55]Martin A: The use of antioxidants in wound healing. Dermatol Surg 1996, 22:156-160.
• [56]Okoli CO, Akah PA, Okoli AS: Potentials of leaves of Aspillia africana (composition) in wound care: an experimental evaluation. BMC Complement Altern Med 2007, 7:24. BioMed Central Full Text