BMC Microbiology | |
Genetic and structural identification of an O-acyltransferase gene (oacC) responsible for the 3/4-O-acetylation on rhamnose III in Shigella flexneri serotype 6 | |
Qiangzheng Sun1  Jianguo Xu1  Nan Zhang1  Alexander S Shashkov2  Pengcheng Du1  Anna M Shpirt2  Ruiting Lan3  Sofya N Senchenkova2  Xia Luo1  Jianping Wang1  Yuriy A Knirel2  | |
[1] State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, China CDC, Changping, Beijing, China;N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation;School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia | |
关键词: Anti-O-factor 9 serum; O-antigen; oacC; Acyltransferase; 3/4-O-acetylation; Shigella flexneri; | |
Others : 1137747 DOI : 10.1186/s12866-014-0266-7 |
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received in 2014-06-30, accepted in 2014-10-09, 发布年份 2014 | |
【 摘 要 】
Background
O-antigen (O-polysaccharide) of the lipopolysaccharide is a highly variable cell component of the outer membrane in Shigella flexneri. It defines the serospecificity and plays an important role in the pathogenesis of shigellosis. There are two distinct O-antigen forms for the 19 serotypes of S. flexneri: one for serotypes 1–5, X, Y, 7 (and their subtypes), and the other for serotype 6. Although having different basal O-polysaccharide structures, the two forms share a common disaccharide fragment [→2)-α-L-RhapIII-(1 → 2)-α-L-RhapII]. In serotype 6 and some non-6 serotypes, RhaIII is O-acetylated at position either 3 or 4 (3/4-O-acetylation), conferring to the hosts a novel antigenic determinant named O-factor 9. An acyltransferase gene (oacB) responsible for this modification has been identified in serotypes 1a, 1b, 2a, 5a, and Y, but not in serotype 6.
Results
Using genetic, serological, and chemical approaches, another acyltransferase gene named oacC was demonstrated to be responsible for the 3/4-O-acetylation on RhaIII in the O-antigen of S. flexneri serotype 6. Inactivation of the oacC gene resulted in the loss of the 3/4-O-acetyltion, and the cloned oacC gene restored this modification upon transformation. In accordance with the similarity in the acceptor substrate structure and high sequence homology (72% identity) between oacC and oacB, oacC has the interchangeable function with the oacB gene in mediation of the 3/4-O-acetylation. The oacC gene is located in a prophage on the chromosome and presented in all 77 serotype 6 strains tested.
Conclusions
Identification and functional characterization of the O-acetyltransferase encoding gene, oacC, shows that it is involved in O-antigen modification by 3/4-O-acetylation on RhaIII specific to serotype 6.
【 授权许可】
2014 Knirel et al.; licensee BioMed Central Ltd.
【 预 览 】
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