Gastroprotective mechanism of Paederia foetida Linn. (Rubiaceae) – a popular edible plant used by the tribal community of North-East India

BMC Complementary and Alternative Medicine

Sayeed Ahmad¹  Kuldeep Singh³  Raj Kumar Tiwari³  Lokesh Deb²  Silpi Chanda³ 

[1] Department of Pharmacognosy and Phytochemistry, Jamia Hamdard, New Delhi 110062, India;Pharmacology Laboratory, Medicinal Plants and Horticultural Resources Division, Institute of Bioresources and Sustainable Development (Department of Biotechnology, Government of India), Takyelpat Institutional Area, Imphal 795001, Manipur, India;Department of Pharmacy, Jaypee University of Information Technology (JUIT), Wakhnaghat, Solan, Himachalpradesh 173234, India

关键词: North-East India;    Antioxidant;    Gastroprotective;    Paederia foetida;   

Others  :  1223705
DOI  :  10.1186/s12906-015-0831-0

 received in 2014-07-18, accepted in 2015-08-26,  发布年份 2015

【 摘 要 】

Background

The objective of the present study is to evaluate the gastroprotective activity of Paederia foetida L. leaf for gastric ulcer.

Methods

The methanol extract of P. foetida L. leaves at two different dose levels was investigated for gastroprotective potential by using Indomethacin-pylorus ligation, alcohol induced and water immersion stress induced model in rats. In vitro DPPH* radical scavenging activity and western blot analysis of stomach tissue from pylorus ligatures rats were also carried out. HPTLC analysis was done to understand the phyto-pharmacological relationship.

Results

Methanol extract at a dose level of 100 mg/kg and 200 mg/kg body weight showed 72 and 78 % ulcer protection when compared to negative control whereas reference drug shown 82 % protection in Indomethacin-pylorus ligation model. Further, methanol extract also showed protective effect against 70 % v/v ethanol and stress induced gastric ulcer model. About 84 % protection as compared to cimetidine (85 %) was seen in western blot analysis of stomach tissue from pylorus ligatures rats. HPTLC analysis of methanol extract of P. foetida L. confirmed the presence of β-sitosterol. In DPPH* radical scavenging activity, the IC ₅₀value was observed to be 43.52 μg/ml.

Conclusions

These observations established the traditional claim and thus Paederia foetida could be a potent gastroprotective agent for use in future. The gastroprotective activity might be mediated by the Nrf2 mediated antioxidant and anti secretory effects.

【 授权许可】

   
2015 Chanda et al.

【 图 表 】

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