The Eucalyptus terpene synthase gene family

BMC Genomics

William J Foley⁵  Jörg Degenhardt⁴  Alexander A Myburg¹  Tobias G Köllner²  Sandra T Krause⁴  Charles Hefer³  Amanda Padovan⁵  Carsten Külheim⁵ 

[1] Department of Genetics, Forestry and Agricultural Biotechnology Institute, Private Bag X20, Pretoria 0028, South Africa;Department of Biochemistry, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany;Department of Botany, University of British Columbia, Vancouver V6T1Z4, BC, Canada;Institut für Pharmazie, Martin-Luther Universität Halle-Wittenberg, Halle, 06120, (Saale), Germany;Research School of Biology, College of Medicine, Biology and the Environment, Australian National University, Canberra 0200, Australia

关键词: Herbivory;    Biodiversity;    Evolution;    Sesquiterpenes;    Monoterpenes;    Essential oil;    Terpene synthase;    Myrtaceae;    Eucalyptus;   

Others  :  1211958
DOI  :  10.1186/s12864-015-1598-x

 received in 2014-12-17, accepted in 2015-04-28,  发布年份 2015

【 摘 要 】

Background

Terpenoids are abundant in the foliage of Eucalyptus, providing the characteristic smell as well as being valuable economically and influencing ecological interactions. Quantitative and qualitative inter- and intra- specific variation of terpenes is common in eucalypts.

Results

The genome sequences of Eucalyptus grandis and E. globulus were mined for terpene synthase genes (TPS) and compared to other plant species. We investigated the relative expression of TPS in seven plant tissues and functionally characterized five TPS genes from E. grandis. Compared to other sequenced plant genomes, Eucalyptus grandis has the largest number of putative functional TPS genes of any sequenced plant. We discovered 113 and 106 putative functional TPS genes in E. grandis and E. globulus, respectively. All but one TPS from E. grandis were expressed in at least one of seven plant tissues examined. Genomic clusters of up to 20 genes were identified. Many TPS are expressed in tissues other than leaves which invites a re-evaluation of the function of terpenes in Eucalyptus.

Conclusions

Our data indicate that terpenes in Eucalyptus may play a wider role in biotic and abiotic interactions than previously thought. Tissue specific expression is common and the possibility of stress induction needs further investigation. Phylogenetic comparison of the two investigated Eucalyptus species gives insight about recent evolution of different clades within the TPS gene family. While the majority of TPS genes occur in orthologous pairs some clades show evidence of recent gene duplication, as well as loss of function.

【 授权许可】

   
2015 Külheim et al.; licensee BioMed Central.

【 图 表 】

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