Electro-acupuncture decreases 5-HT, CGRP and increases NPY in the brain-gut axis in two rat models of Diarrhea-predominant irritable bowel syndrome(D-IBS)

BMC Complementary and Alternative Medicine

Wei Zhang⁵  Lixia Pei¹  Yongjun Peng¹  Houxu Ning³  Jie Cheng⁵  Yunfang Meng⁴  Xiaoliang Wu²  Jianhua Sun¹ 

[1]Department of Acupuncture and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China

[2]Chinese Medicine Hospital of Jiangsu Province, 155 Hanzhong Road, Nanjing 210029, Jiangsu Province, China

[3]Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing 210029, Jiangsu Province, China

[4]Shanghai Key Laboratory of Molecular Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China

[5]Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China

关键词: Neuro-peptide Y (NPY);    Calcitonin gene-related peptide (CGRP);    5-hydroxytryptamine (5-HT);    Electro-acupuncture (EA);    Brain-gut axis (BGA);    Diarrhea-predominant irritable bowel syndrome (D-IBS);   

Others  :  1233544
DOI  :  10.1186/s12906-015-0863-5

 received in 2015-05-29, accepted in 2015-09-16,  发布年份 2015

【 摘 要 】

Background

To examine whether electro-acupuncture (EA) could decrease 5-hydroxytryptamine (5-HT) and calcitonin gene-related peptide (CGRP), and increase neuro-peptide Y (NPY) in the brain-gut axis (BGA) in D-IBS using rat models.

Methods

Rats were randomly exposed to unpredictable chronic stress for 3 weeks followed by 1-hour acute restraint stress (CAS) after 7 days of rest, or daily gavage of Senna decoction (6 g/kg) plus chronic restraint stress (for a duration of 2 h, starting from 1 h prior to the gavage) for 2 weeks (ISC). The content of 5-HT, CGRP and NPY in the distal colon, spinal cord, hypothalamus was examined at the end of the treatment.

Results

1. The two rat models exhibited similar characteristics, e.g., increased number of fecal pellets expelled in 1 h, decreased sacchar-intake, decreased CRD, elevated 5-HT, CGRP content and decreased NPY in the distal colon, spinal cord, hypothalamus (P < 0.05 vs. that in healthy control rats). 2. A series of equations was developed based on correlation regression analysis. The analysis results demonstrated that 5-HT mediates the changes in hypothalamus, spinal cord and colon. 5-HT and CGRP in spinal cord was closely correlated with general behavior evaluation and other transmitters in BGA.

Conclusion

1. In comparison to 5-HT, CGRP and NPY (particularly in the spinal cord) had closer relationship with the D-IBS symptoms induced by either stress factors or Senna decotion.

2. EA treatment could restore the brain-gut axis to balanced levels.

【 授权许可】

   
2015 Sun et al.

【 图 表 】

Fig. 1.

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