Directed evolution of cell size in Escherichia coli

BMC Evolutionary Biology

Tetsuya Yomo³  Bei-Wen Ying²  Hideo Matsuda¹  Shigeto Seno¹  Naoko Hirata¹  Saburo Tsuru¹  Mari Yoshida¹ 

[1]Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita 565-0871, Osaka, Japan

[2]Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, Japan

[3]Earth-Life Science Institute, Tokyo Institute of Technology, 2-12-1-IE-1 Ookayama, Meguro-ku 152-8550, Tokyo, Japan

关键词: Evolutional constraints;    Experimental evolution;    Cell size;   

Others  :  1121754
DOI  :  10.1186/s12862-014-0257-1

 received in 2014-07-31, accepted in 2014-11-27,  发布年份 2014

【 摘 要 】

Background

In bacteria, cell size affects chromosome replication, the assembly of division machinery, cell wall synthesis, membrane synthesis and ultimately growth rate. In addition, cell size can also be a target for Darwinian evolution for protection from predators. This strong coupling of cell size and growth, however, could lead to the introduction of growth defects after size evolution. An important question remains: can bacterial cell size change and/or evolve without imposing a growth burden?

Results

The directed evolution of particular cell sizes, without a growth burden, was tested with a laboratory Escherichia coli strain. Cells of defined size ranges were collected by a cell sorter and were subsequently cultured. This selection-propagation cycle was repeated, and significant changes in cell size were detected within 400 generations. In addition, the width of the size distribution was altered. The changes in cell size were unaccompanied by a growth burden. Whole genome sequencing revealed that only a few mutations in genes related to membrane synthesis conferred the size evolution.

Conclusions

In conclusion, bacterial cell size could evolve, through a few mutations, without growth reduction. The size evolution without growth reduction suggests a rapid evolutionary change to diverse cell sizes in bacterial survival strategies.

【 授权许可】

   
2014 Yoshida et al.; licensee BioMed Central.

【 图 表 】

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