Characterization of adipose-derived stem cells of anatomical region from mice

BMC Research Notes

Durvanei A Maria¹  Rosa AN Laiso²  José ALC Moreira²  Thais O Conceição²  Maria EP Madeira¹  Arthur CL Luna¹ 

[1] Medical School, University of Sao Paulo, 455, Doctor Arnaldo Avenue, Sao Paulo 01246-903, Brazil;Biochemistry and Biophysical Laboratory, Butantan Institute, 1500, Vital Brasil Avenue, Sao Paulo, Brazil

关键词: Mice;    Mesenchymal stem cells;    Multipotent cells;    Cyclin D1;    Caspase-3;    Adipose tissue;   

Others  :  1130339
DOI  :  10.1186/1756-0500-7-552

 received in 2013-12-17, accepted in 2014-08-14,  发布年份 2014

【 摘 要 】

Background

Stem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential. Several studies have provided evidence that adipose tissue represents an alternative source of stem cells, with the main benefit of adipose-derived stem cells being that they can be easily harvested from patients by a simple minimally invasive method and can be easily cultured. The aim of this study was to establish a culture protocol for obtaining and characterizing adipose-derived stem cells (ADSCs) from C57BL/6 J mice.

Results

The results showed that the yield, viability, and cell morphology obtained differ according to the age of isolated anatomic regions of the adipose tissue from ovarian and epididymis. The results of determination of cyclin D1 showed uniformity in the expression between different populations of ADSCs. A significant increase in the expression of caspase-3 active, was also observed in large cell populations from mice after 120 days. ADSCs were positive for mesenchymal markers CD90 and CD105, Nanog, SSEA-1, CD106, and VEGFR-1, and negative for hematopoietic markers CD34 and CD45. A large number of cells in S + G2/M phases was also observed for both sexes, demonstrating high proliferative capacity of ADSCs.

Conclusions

We observed that the adipose tissue of C57BL/6 J mice, isolated from the studied anatomic regions, is a promising source for obtaining pluripotent mesenchymal stem cells with high viability and proliferative response.

【 授权许可】

   
2014 Luna et al.; licensee BioMed Central Ltd.

【 图 表 】

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