L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences

BMC Microbiology

Toril Lindbäck¹  Janet M Blatny²  Per Einar Granum¹  Elisabeth H Madslien¹ 

[1] Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, P. O. Box 8146 Dep, N-0033 Oslo, Norway;Forsvarets Forskningsinstitutt FFI, Norwegian Defence Research Establishment, P. O. Box 25, N-2027 Kjeller, Norway

关键词: Germinant receptor;    Genotype;    gerA;    L-alanine;    Germination;    Bacillus licheniformis;   

Others  :  1141347
DOI  :  10.1186/1471-2180-14-101

 received in 2014-01-28, accepted in 2014-04-09,  发布年份 2014

【 摘 要 】

Background

L-alanine, acting through the GerA receptor, was recently found to be an efficient germinant in Bacillus licheniformis ATCC14580/DSM13.

Results

In this study, we show that several of 46 examined B. licheniformis strains germinate remarkably slower than the type strain when exposed to L-alanine. These strains are not necessarily closely related, as determined by MLST (multi-locus sequence typing). Three of the slow-germinating strains were further examined in order to see whether nucleotide substitutions in the gerA sequences were responsible for the slow L-alanine germination. This was performed by complementing the transformable type strain derivate MW3ΔgerAA with gerA variants from the three slow-germinating strains; NVH1032, NVH1112 and NVH800.

Conclusions

A wide selection of B. licheniformis strains was evaluated for L-alanine-induced germination efficiency. Our results show that gerA substitutions could only partially explain why spores of some B. licheniformis strains responded slower than others in the presence of L-alanine.

【 授权许可】

   
2014 Madslien et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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