Molecular typing and characterization of nasal carriage and community-onset infection methicillin-susceptible Staphylococcus aureus isolates in two Taiwan medical centers

BMC Infectious Diseases

Po-Liang Lu³  Chen-Her Wang²  Jung-Chung Lin¹  Leung-Kei Kristopher Siu⁴  Feng-Jui Chen² 

[1] Department of Internal Medicine, Division of Infectious Diseases and Tropical Medicine, Tri-Service General Hospital, Taipei, Taiwan;Division of Infectious Diseases, National Health Research Institutes, Zhunan, Taiwan;School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan

关键词: Spa typing;    Multi-locus sequence typing;    Pulsed-field gel electrophoresis;    Lineage;    Staphylococcus aureus;    MSSA;    Infection;    Community onset;    Nasal carriage;   

Others  :  1158728
DOI  :  10.1186/1471-2334-12-343

 received in 2012-06-08, accepted in 2012-12-03,  发布年份 2012

【 摘 要 】

Background

Compared to methicillin-resistant Staphylococcus aureus (MRSA), characteristics of nasal carriage and community-onset infection methicillin-susceptible S. aureus (MSSA) are less well known. No characteristics of MSSA in Taiwan have been reported previously.

Methods

We analyzed 100 nasal carriage and 34 community-onset infection MSSA isolates by pulsed-field gel electrophoresis (PFGE), spa typing, multi-locus sequence typing, agr typing, virulence gene detection, growth rate measurement, and antimicrobial susceptibility.

Results

In PFGE analysis, most (68%) infection isolates could be grouped in one major cluster using a 70% similarity cutoff. In contrast, only 17% of nasal carriage isolates belonged to this cluster. A similar classification was obtained using Based Upon Repeat Pattern analysis of spa types. The MSSA infection isolates cluster was closely related to the virulent clones of clonal complex 1 (CC1), which includes strains MW2 (USA400) and MSSA476. ST188 of CC1 was the predominant clone detected for community-onset MSSA infections. The only common ST type for MSSA and MRSA in Taiwan was ST59, the community-associated MRSA clone. It is likely, therefore, that MRSA originated from MSSA clones through SCCmec transfer. Compared to nasal carriage isolates, infection isolates less frequently possessed egc, tst and hlg genes, were more commonly susceptible to erythromycin (91% vs. 54%), and had shorter mean doubling times (38 min vs. 55 min).

Conclusions

The clonal lineages of MSSA nasal carriage and infection isolates differed in our sample of Taiwan isolates. Most community-onset MSSA infections resulted from relatively few clonal lineages. Nasal carriage isolates more frequently possessed the egc, tst and hlg genes, were more resistant to erythromycin, and grew more slowly.

【 授权许可】

   
2012 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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