A framework to identify gene expression profiles in a model of inflammation induced by lipopolysaccharide after treatment with thalidomide

BMC Research Notes

Flavio F Nobre¹  Euzenir N Sarno²  Ulisses G Lopes³  Elizabeth P Sampaio²  Daniel Serra de Carvalho²  Jorgenilce de Souza Sales²  Tatiana O Fulco²  Alessandra M Saliba⁴  Renata T Paiva¹ 

[1]Biomedical Engineering Program, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

[2]Leprosy Laboratory, Institute Oswaldo Cruz, Rio de Janeiro, Brazil

[3]Department of Molecular and Structural Biology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

[4]Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

关键词: Lipopolysaccharide;    Inflammation model;    Rank product;    Microarray;    Thalidomide;   

Others  :  1166311
DOI  :  10.1186/1756-0500-5-292

 received in 2012-04-06, accepted in 2012-05-30,  发布年份 2012

【 摘 要 】

Background

Thalidomide is an anti-inflammatory and anti-angiogenic drug currently used for the treatment of several diseases, including erythema nodosum leprosum, which occurs in patients with lepromatous leprosy. In this research, we use DNA microarray analysis to identify the impact of thalidomide on gene expression responses in human cells after lipopolysaccharide (LPS) stimulation. We employed a two-stage framework. Initially, we identified 1584 altered genes in response to LPS. Modulation of this set of genes was then analyzed in the LPS stimulated cells treated with thalidomide.

Results

We identified 64 genes with altered expression induced by thalidomide using the rank product method. In addition, the lists of up-regulated and down-regulated genes were investigated by means of bioinformatics functional analysis, which allowed for the identification of biological processes affected by thalidomide. Confirmatory analysis was done in five of the identified genes using real time PCR.

Conclusions

The results showed some genes that can further our understanding of the biological mechanisms in the action of thalidomide. Of the five genes evaluated with real time PCR, three were down regulated and two were up regulated confirming the initial results of the microarray analysis.

【 授权许可】

   
2012 Paiva et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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