Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins

BMC Genomics

Augusto Schrank²  Ana Tereza Ribeiro de Vasconcelos¹  Marilene Henning Vainstein²  Lívia Kmetzsch²  Eder Silva de Oliveira²  Thaiane Rispoli Serrano²  Carolina Pereira Silveira²  Walter Orlando Beys-da-Silva²  Lucélia Santi²  Melissa Landell²  Leonardo Broetto²  Rana Louise de Andrade da Paixão²  Nicolau Sbaraini²  Alexandra Lehmkuhl Gerber¹  Fábio Carrer Andreis²  Luiz Gonzaga Paula de Almeida¹  Juliano Tomazzoni Boldo²  Guilherme Loss de Morais¹  Claudia Elizabeth Thompson²  Rafael Lucas Muniz Guedes¹  Ângela Junges²  Charley Christian Staats² 

[1] Laboratório Nacional de Computação Científica (LNCC), Av. Getúlio Vargas, 333, Petrópolis, RJ, Brazil;Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), P. O. Box 15005, Porto Alegre, RS CEP 91501-970, Brazil

关键词: Phylogenomics;    Secretome;    Entomopathogenic fungi;    Genome sequence;   

Others  :  1139509
DOI  :  10.1186/1471-2164-15-822

 received in 2014-06-04, accepted in 2014-08-29,  发布年份 2014

【 摘 要 】

Background

Metarhizium anisopliae is an entomopathogenic fungus used in the biological control of some agricultural insect pests, and efforts are underway to use this fungus in the control of insect-borne human diseases. A large repertoire of proteins must be secreted by M. anisopliae to cope with the various available nutrients as this fungus switches through different lifestyles, i.e., from a saprophytic, to an infectious, to a plant endophytic stage. To further evaluate the predicted secretome of M. anisopliae, we employed genomic and transcriptomic analyses, coupled with phylogenomic analysis, focusing on the identification and characterization of secreted proteins.

Results

We determined the M. anisopliae E6 genome sequence and compared this sequence to other entomopathogenic fungi genomes. A robust pipeline was generated to evaluate the predicted secretomes of M. anisopliae and 15 other filamentous fungi, leading to the identification of a core of secreted proteins. Transcriptomic analysis using the tick Rhipicephalus microplus cuticle as an infection model during two periods of infection (48 and 144 h) allowed the identification of several differentially expressed genes. This analysis concluded that a large proportion of the predicted secretome coding genes contained altered transcript levels in the conditions analyzed in this study. In addition, some specific secreted proteins from Metarhizium have an evolutionary history similar to orthologs found in Beauveria/Cordyceps. This similarity suggests that a set of secreted proteins has evolved to participate in entomopathogenicity.

Conclusions

The data presented represents an important step to the characterization of the role of secreted proteins in the virulence and pathogenicity of M. anisopliae.

【 授权许可】

   
2014 Staats et al.; licensee BioMed Central Ltd.

【 图 表 】

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