Effect of the fragrance inhalation of essential oil from Asarum heterotropoides on depression-like behaviors in mice

BMC Complementary and Alternative Medicine

Chae Ha Yang⁵  Young Seob Gwak⁵  Kwang Joong Kim⁵  Hyun Soo Shim²  Suchan Chang⁵  Hee Young Kim⁵  Jin Suk Koo⁴  Eun Sook Lee⁵  Eun-Mi Ahn⁵  Ji Wook Jung⁵  Sa Rang Oh⁵  Rong Jie Zhao³  Eun-Ju Lim⁵  Hyun-Jung Park¹ 

[1]Bringham Young University, Provo 84602, Utah, USA

[2]Kyung Hee University, Seoul 130-701, South Korea

[3]Mudanjiang Medical University, Mudanjiang 157011, China

[4]Andong National University, Andong 760-749, Gyeongsangbuk-do, South Korea

[5]College of Korean Medicine, Daegu Hanny University, Daegu 706-828, South Korea

关键词: Inhalation;    Depression;    Asarum heterotropoides;    Essential oil;   

Others  :  1136441
DOI  :  10.1186/s12906-015-0571-1

 received in 2014-10-14, accepted in 2015-02-21,  发布年份 2015

【 摘 要 】

Background

Psychological stressors may cause affective disorders, such as depression and anxiety, by altering expressions of corticotropin releasing factor (CRF), serotonin (5-HT), and tyrosine hydroxylase (TH) in the brain. This study investigated the effects of essential oil from Asarum heterotropoides (EOAH) on depression-like behaviors and brain expressions of CRF, 5-HT, and TH in mice challenged with stress.

Methods

Male ICR mice received fragrance inhalation of EOAH (0.25, 0.5, 1.0, and 2.0 g) for 3 h in the special cage capped with a filter paper before start of the forced swimming test (FST) and tail suspension test (TST). The duration of immobility was measured for the determination of depression-like behavior in the FST and TST. The selective serotonin reuptake inhibitor fluoxetine as positive control was administered at a dose of 15 mg/kg (i.p.) 30 min before start of behavioral testing. Immunoreactivities of CRF, 5-HT, and TH in the brain were also measured using separate groups of mice subjected to the FST.

Results

EOAH at higher doses (1.0 and 2.0 g) reduced immobility time in the FST and TST. In addition, EOAH at a dose of 1.0 g significantly reduced the expected increases in the expression of CRF positive neurons in the paraventricular nucleus and the expression of TH positive neurons in the locus coeruleus, and the expected decreases of the 5-HT positive neurons in the dorsal raphe nucleus.

Conclusion

These results provide strong evidence that EOAH effectively inhibits depression-like behavioral responses, brain CRF and TH expression increases, and brain 5-HT expression decreases in mice challenged with stress.

【 授权许可】

   
2015 Park et al.; licensee BioMed Central.

【 图 表 】

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