Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells

BMC Cancer

Harue Akasaka¹  Fuyuki Sato¹  Satoko Morohashi¹  Yunyan Wu²  Yang Liu²  Jun Kondo¹  Hiroki Odagiri³  Kenichi Hakamada³  Hiroshi Kijima¹ 

[1] Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan

[2] Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang, 110001, China

[3] Department of Surgery, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan

Others  :  1081283
DOI  :  10.1186/1471-2407-10-548

 received in 2010-05-08, accepted in 2010-10-12,  发布年份 2010

【 摘 要 】

Background

Claudin-1 is a membrane protein of tight junctions, and is associated with the development of various cancers. However, the significance of claudin-1 expression in cancer cells is not well understood. Here, we showed for the first time the anti-apoptotic effect of claudin-1 in human breast cancer MCF-7 cells.

Methods

Human breast cancer MCF-7 and T47 D cells were treated with or without tamoxifen, siRNA against claudin-1, or tamoxifen and claudin-1 siRNA. The samples were analyzed by RT-PCR, Western blotting or immunofluorescent staining.

Results

The expression of claudin-1 was upregulated in tamoxifen-treated MCF-7 cells, whereas the expression of claudin-1 was not altered in tamoxifen-treated T47 D cells. Knockdown of claudin-1 by siRNA increased the amount of poly (ADP-ribose) polymerase (PARP) regardless of tamoxifen treatment in MCF-7 cells, but not T47 D cells. In the cell membranes of the MCF-7 cells, tamoxifen treatment increased the amount of claudin-1, but decreased the amount of β-catenin. Claudin-1 siRNA increased the amount of E-cadherin in the cytoplasm of the MCF-7 cells as well as the amount of β-catenin in their cell membranes.

Conclusion

These results indicate that claudin-1 has anti-apoptotic effects, and is involved in the regulation of the expression and subcellular localization of β-catenin and E-cadherin in MCF-7, but not T47 D cells.

【 授权许可】

   
2010 Akasaka et al; licensee BioMed Central Ltd.

【 图 表 】

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