Evolution of Escherichia coli rifampicin resistance in an antibiotic-free environment during thermal stress

BMC Evolutionary Biology

Olivier Tenaillon¹  Brandon S Gaut³  Alejandra Rodríguez-Verdugo² 

[1] INSERM UMR-S 722, Université Paris7, Faculté de Médicine Denis Diderot, Site Xavier Bichat, 16 rue Henri Huchard, 75018, Paris, France;INSERM, Université Paris7, Faculté de Médicine Denis Diderot, UMR-S 722, Paris, France;Department of Ecology and Evolutionary Biology, UC Irvine, Irvine, CA, USA

关键词: Epistasis;    Pleiotropy;    Trade-offs;    Experimental evolution;    Fitness effects;    Beneficial mutations;   

Others  :  1129778
DOI  :  10.1186/1471-2148-13-50

 received in 2012-11-06, accepted in 2013-01-11,  发布年份 2013

【 摘 要 】

Background

Beneficial mutations play an essential role in bacterial adaptation, yet little is known about their fitness effects across genetic backgrounds and environments. One prominent example of bacterial adaptation is antibiotic resistance. Until recently, the paradigm has been that antibiotic resistance is selected by the presence of antibiotics because resistant mutations confer fitness costs in antibiotic free environments. In this study we show that it is not always the case, documenting the selection and fixation of resistant mutations in populations of Escherichia coli B that had never been exposed to antibiotics but instead evolved for 2000 generations at high temperature (42.2°C).

Results

We found parallel mutations within the rpoB gene encoding the beta subunit of RNA polymerase. These amino acid substitutions conferred different levels of rifampicin resistance. The resistant mutations typically appeared, and were fixed, early in the evolution experiment. We confirmed the high advantage of these mutations at 42.2°C in glucose-limited medium. However, the rpoB mutations had different fitness effects across three genetic backgrounds and six environments.

Conclusions

We describe resistance mutations that are not necessarily costly in the absence of antibiotics or compensatory mutations but are highly beneficial at high temperature and low glucose. Their fitness effects depend on the environment and the genetic background, providing glimpses into the prevalence of epistasis and pleiotropy.

【 授权许可】

   
2013 Rodríguez-Verdugo et al; licensee BioMed Central Ltd.

【 图 表 】

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