Immune cell transcript modules reveal leukocyte heterogeneity in synovial biopsies of seronegative spondylarthropathy patients

BMC Musculoskeletal Disorders

Tracy Chew²  Robin M Hallett¹ 

[1] Path Genomics, Hamilton, Ontario, Canada;Department of Pathology and Microbiology, Canadian Memorial Chiropractic College, 6100 Leslie Street, Toronto, Ontario M2H 3J1, Canada

关键词: Computational biology;    Metagene;    Gene signature;    Transcriptional analysis;    Leukocyte;    Rheumatology;    Inflammation;    Immune infiltration;    Ankylosing spondylitis;    Spondylarthropathies;   

Others  :  1090257
DOI  :  10.1186/1471-2474-15-446

 received in 2014-06-26, accepted in 2014-12-16,  发布年份 2014

【 摘 要 】

Background

The objective of this study was to identify leukocyte cell types found within the synovia of patients with seronegative spondylarthropathies (SpA), such as ankylosing spondylitis (AS), using transcription based analyses.

Methods

Leukocyte transcriptional profiles obtained from the NCBI’s gene expression omnibus and prediction analysis of microarrays (PAM) was used to identify 25-gene leukocyte metagenes. Subsequently, transcriptional profiles from murine and clinical models of AS and SpA were interrogated to characterize the local infiltration of leukocytes in SpA synovia.

Results

Analysis of a proteoglycan-induced murine model of AS reveals infiltration of dendritic cells, CD4+ T cells, monocytes, and natural killer cells to the spine. In human SpA and AS patients, transcriptional analysis of synovial biopsies revealed local infiltration of dendritic cells and CD4+ T cells.

Conclusions

We identified leukocyte cell types that infiltrated the synovial of SpA patients. Our results imply a role for dendritic cells and CD4+ T cells in the local inflammation that underlies pathogenesis in patients with SpA.

【 授权许可】

   
2014 Hallett and Chew; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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