Evidence-based antioxidant activity of the essential oil from Fructus A. zerumbet on cultured human umbilical vein endothelial cells’ injury induced by ox-LDL

BMC Complementary and Alternative Medicine

Yu-yi Xia²  Hong Luo²  Yan-yan Zhang²  Wan-kui Li²  Ling Tao¹  Xiang-chun Shen² 

[1] Department of Pharmaceutics, Guiyang Medical University, Guiyang 55004, People’s Republic of China;Research Division of Pharmacology, Guiyang Medical University, Guiyang 55004, People’s Republic of China

关键词: Oxidative stress;    Oxidized low-density lipoprotein;    Human umbilical vein endothelial cells;    Fructus Alpiniae zerumbet;    Essential oil;   

Others  :  1231951
DOI  :  10.1186/1472-6882-12-174

 received in 2012-05-12, accepted in 2012-10-05,  发布年份 2012

【 摘 要 】

Background

The essential oil from Fructus Alpiniae zerumbet (FAZ) is its principal bioactive ingredient, and is widely used in Miao folk herbs in Guizhou province for the treatment of gastrointestinal and cardiovascular diseases. Several studies have confirmed that FAZ ameliorates hyperlipidemia and atherosclerosis. Because endothelial dysfunction often accompanies cardiovascular diseases, especially hyperlipidemia and atherosclerosis, the present study concentrated on evaluating the endothelial protective effects of the essential oil from FAZ (EOFAZ) on oxidized low-density lipoprotein (ox-LDL)-induced injury of cultured human umbilical vein endothelial cells (HUVECs) and on the regulation of oxidative stress.

Methods

Cell viability was analyzed with the MTT assay and trypan blue exclusion staining (TBES). Cell injury was assessed by lactate dehydrogenase (LDH) release. Biochemical enzymatic methods were used to evaluate the oxidative stress, including the lipid peroxidation product, malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px).

Results

The redox status of HUVECs was significantly exacerbated after exposure to ox-LDL. EOFAZ protected HUVECs against ox-LDL injury as assessed by the MTT assay, TBES and LDH release. Furthermore, EOFAZ ameliorated the oxidative stress by elevating the activities of SOD, CAT and GSH-Px, and increasing the GSH levels, in addition to attenuating the MDA contents.

Conclusions

The present data provide the first experimental evidence that EOFAZ protects endothelial cells against ox-LDL-induced injury, and indicate that this protection involves ameliorating the redox status.

【 授权许可】

   
2012 Shen et al.; licensee BioMed Central Ltd.

【 图 表 】

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