miRNA regulated pathways in late stage murine lung development

BMC Developmental Biology

Heber C Nielsen⁴  MaryAnn V Volpe¹  Tanya Logvinenko²  Sana Mujahid³ 

[1] Division of Newborn Medicine, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, 800 Washington Street, Boston, MA, 02111, USA;Institute for Clinical Research and Health Policy Studies, Tufts Medical Center, Boston, MA, USA;Program in Cell, Molecular and Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA, USA;Department of Anatomy and Cell Biology Tufts University School of Medicine, Boston, MA, USA

关键词: Profiling;    Sex;    Gestation;    microRNA;   

Others  :  1085934
DOI  :  10.1186/1471-213X-13-13

 received in 2012-10-12, accepted in 2013-04-16,  发布年份 2013

【 摘 要 】

Background

MicroRNAs play important roles in regulating biological processes, including organ morphogenesis and maturation. However, little is known about specific pathways regulated by miRNA during lung development. Between the canalicular and saccular stages of the developing lung several important cellular events occur, including the onset of surfactant synthesis, microvascular remodeling and structural preparation for subsequent alveolarization. The miRNAs that are actively regulated, and the identity of their targets during this important developmental interval in the lung remain elusive.

Results

Using TLDA low density real-time PCR arrays, the expression of 376 miRNAs in male and female fetal mouse lungs of gestational days E15 – E18 were profiled. Statistical analyses identified 25 and 37 miRNAs that changed significantly between sexes and with gestation, respectively. In silico analysis using Ingenuity Pathway Analysis (IPA) identified specific pathways and networks known to be targets of these miRNAs which are important to lung development. Pathways that are targeted by sex regulated miRNAs include retinoin, IGFR1, Tp53 and Akt. Pathways targeted by gestation-regulated miRNAs include VEGFA and mediators of glucose metabolism.

Conclusion

MiRNAs are differentially regulated across time and between sexes during the canalicular and saccular stages of lung development. Sex-associated differential miRNA expression may regulate the differences in structural and functional male and female lung development, as shown by networks generated using in silico analysis. These data provide a valuable resource to further enhance the understanding of miRNA control of lung development and maturation.

【 授权许可】

   
2013 Mujahid et al.; licensee BioMed Central Ltd.

【 图 表 】

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