Competition between Burkholderia pseudomallei and B. thailandensis

BMC Microbiology

Narisara Chantratita⁴  Sharon J Peacock²  David A Low³  Jeanne Salje⁴  Vanaporn Wuthiekanun⁴  Onrapak Reamtong¹  Chanthiwa Wikraiphat⁴  Sarunporn Tandhavanant⁴  Wikanda Ngamdee⁵ 

[1] Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK;Biomolecular Science and Engineering Program, University of California, Santa Barbara, CA, USA;Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand

关键词: Competitive growth inhibition;    Flagella;    Swarming;    Melioidosis;    B. thailandensis;    Burkholderia pseudomallei;   

Others  :  1135405
DOI  :  10.1186/s12866-015-0395-7

 received in 2014-12-08, accepted in 2015-02-19,  发布年份 2015

【 摘 要 】

Background

Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, an often fatal disease in tropical countries. Burkholderia thailandensis is a non-virulent but closely related species. Both species are soil saprophytes but are almost never isolated together.

Results

We identified two mechanisms by which B. pseudomallei affects the growth of B. thailandensis. First, we found that six different isolates of B. pseudomallei inhibited the growth of B. thailandensis on LB agar plates. Second, our results indicated that 55% of isolated strains of B. pseudomallei produced a secreted compound that inhibited the motility but not the viability of B. thailandensis. Analysis showed that the active compound was a pH-sensitive and heat-labile compound, likely a protein, which may affect flagella processing or facilitate their degradation. Analysis of bacterial sequence types (STs) demonstrated an association between this and motility inhibition. The active compound was produced from B. pseudomallei during the stationary growth phase.

Conclusion

Taken together, our results indicate that B. pseudomallei inhibits both the growth and motility of its close relative B. thailandensis. The latter phenomenon appears to occur via a previously unreported mechanism involving flagellar processing or degradation.

【 授权许可】

   
2015 Ngamdee et al.; licensee BioMed Central.

【 图 表 】

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