The genome of the Lactobacillus sanfranciscensis temperate phage EV3

BMC Research Notes

Rudi F Vogel¹  Roberto Foschino³  Claudia Picozzi³  Angel Angelov²  Matthias A Ehrmann¹ 

[1]Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel-Str. 4, Freising 85354, Germany

[2]Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann-Str. 4, Freising 85354, Germany

[3]Dipartimento di Scienze per gli Alimenti, la Nutrizione e l’Ambiente, Università degli Studi di Milano, DeFENS, via Celoria, Milano 2 – 20133, Italy

关键词: Sourdough fermentation;    Lactobacillus sanfranciscensis;    EMBL accession number PRJEB61;    Bacteriophage;    Genome;   

Others  :  1140543
DOI  :  10.1186/1756-0500-6-514

 received in 2013-09-02, accepted in 2013-11-29,  发布年份 2013

【 摘 要 】

Background

Bacteriophages infection modulates microbial consortia and transduction is one of the most important mechanism involved in the bacterial evolution. However, phage contamination brings food fermentations to a halt causing economic setbacks. The number of phage genome sequences of lactic acid bacteria especially of lactobacilli is still limited. We analysed the genome of a temperate phage active on Lactobacillus sanfranciscensis, the predominant strain in type I sourdough fermentations.

Results

Sequencing of the DNA of EV3 phage revealed a genome of 34,834 bp and a G + C content of 36.45%. Of the 43 open reading frames (ORFs) identified, all but eight shared homology with other phages of lactobacilli. A similar genomic organization and mosaic pattern of identities align EV3 with the closely related Lactobacillus vaginalis ATCC 49540 prophage. Four unknown ORFs that had no homologies in the databases or predicted functions were identified. Notably, EV3 encodes a putative dextranase.

Conclusions

EV3 is the first L. sanfranciscensis phage that has been completely sequenced so far.

【 授权许可】

   
2013 Ehrmann et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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