Adenosine preconditioning attenuates hepatic reperfusion injury in the rat by preventing the down-regulation of endothelial nitric oxide synthase

BMC Gastroenterology

Robert T Mathie¹  Robin CN Williamson¹  Nagy A Habib¹  Ioannis T Virlos¹  Ferdinand Serracino-Inglott¹ 

[1] Division of Surgery, Anaesthetics & Intensive Care, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, UK

关键词: N^G-Nitro-L-arginine;    immunohistochemistry;    Wistar rats;    liver;    ischemia;   

Others  :  1215903
DOI  :  10.1186/1471-230X-2-22

 received in 2002-04-23, accepted in 2002-09-20,  发布年份 2002

【 摘 要 】

Background

Previous work has suggested that in the liver, adenosine preconditioning is mediated by nitric oxide. Whether the endothelial isoform of nitric oxide synthase plays a part in this mechanism has however not yet been investigated.

Methods

Wistar rats were used (6 in each group) – Groups: (1) sham, (2) ischemia-reperfusion, (3) adenosine + ischemia-reperfusion, (4) endothelial isoform inhibitor + adenosine + ischemia-reperfusion.

Results

Using immunohistochemistry, this study has revealed a decrease in the expression of endothelial nitric oxide synthase following hepatic ischemia-reperfusion. This was prevented by adenosine pre-treatment. When an inhibitor of endothelial nitric oxide synthase was administered prior to adenosine pre-treatment, pre-conditioning did not occur despite normal expression of endothelial nitric oxide synthase.

Conclusions

These findings suggest that adenosine attenuates hepatic injury by preventing the downregulation of endothelial nitric oxide synthase that occurs during ischemia-reperfusion.

【 授权许可】

   
2002 Serracino-Inglott et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 图 表 】

Figure 1.

Figure 2.

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