In vitro determination of the anti-aging potential of four southern African medicinal plants

BMC Complementary and Alternative Medicine

Vanessa Steenkamp³  Werner Cordier³  Malefa Tselanyane¹  Gerda Fouche⁴  Gugulethu Ndlovu² 

[1] Molecular and Biomedical Technologies, Biosciences Unit, Council for Scientific and Industrial Research, P.O. Box 395, Pretoria (0001), South Africa;Department of Chemistry, University of Free-State, P.O. Box 339, Bloemfontein (9300), South Africa;Department of Pharmacology, University of Pretoria, Private Bag X323, Arcadia (0007), South Africa;Natural Product Chemistry Group, Biosciences Unit, Council for Scientific and Industrial Research, P.O. Box 395, Pretoria (0001), South Africa

关键词: Medicinal plants;    Anti-oxidant;    Anti-hyaluronidase;    Anti-elastase;    Anti-collagenase;    Anti-aging;   

Others  :  1220811
DOI  :  10.1186/1472-6882-13-304

 received in 2013-06-14, accepted in 2013-10-28,  发布年份 2013

【 摘 要 】

Background

Aging is an inevitable process for all living organisms. During this process reactive oxygen species generation is increased which leads to the activation of hyaluronidase, collagenase and elastase, which can further contribute to skin aging. Four southern African medicinal plants; Clerodendrum glabrum, Schotia brachypetala, Psychotria capensis and Peltophorum africanum, were investigated to assess their anti-aging properties.

Methods

Anti-elastase, anti-collagenase and anti-hyaluronidase activities of twenty-eight samples, consisting of methanol and ethyl acetate extracts of the four plants, were determined using spectrophotometric methods. Radical scavenging activity was determined by the ability of the plant extracts to scavenge the ABTS^•+ radical.

Results

The majority of the samples in the anti-elastase assay and nine in the anti-collagenase assay showed more than 80% inhibition. The ethyl acetate extract of S. brachypetala bark and leaves of P. capensis inhibited elastase activity by more than 90%. The methanol extract of S. brachypetala bark contained the highest anti-hyaluronidase activity (75.13 ± 7.49%) whilst the ethyl acetate extract of P. africanum bark exhibited the highest antioxidant activity (IC₅₀: 1.99 ± 0.23 μg/ml).

Conclusion

The free radical scavenging activity and enzyme inhibitory activity of the plant extracts investigated suggests that they can help restore skin elasticity and thereby slow the wrinkling process. P. africanum was the plant with the most promising activity and will be subjected to further testing and isolation of the active compound/s.

【 授权许可】

   
2013 Ndlovu et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Mukherjee PK, Maity N, Nema NK, Sarkarm BK: Bioactive compounds from natural resources against skin aging. Phytomedicine 2011, 19:64-73.
• [2]Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan J, Data S, Voorhees JJ: Mechanisms of photoaging and chronological skin aging. Arch Dermatol 2002, 138(11):1462-1470.
• [3]Maity N, Nema NK, Abedy S, Sarkar BK, Mukherjee PK: Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity. J Ethnopharmacol 2011, 137:1300-1305.
• [4]Rittie L, Fisher GJ: UV-light-induced signal cascades and skin aging. Ageing Res Rev 2002, 1:705-720.
• [5]Fulop T, Khalil A, Larbi A: The role of elastin peptides in modulating the immune response in aging and age-related diseases. Pathol Biol 2012, 60:28-33.
• [6]Kurtz A, Oh S-J: Age related changes of the extracellular matrix and stem cell maintenance. Prev Med 2012, 54:S50-S56.
• [7]Wary KK, Thakker GD, Humtsoe JO, Yang J: Analysis of VEGF-responsive genes involved in the activation of endothelial cells. Mol Cancer 2003, 2:25-36. BioMed Central Full Text
• [8]Losso JN, Munene CN, Bansode RR, Bawadi HA: Inhibition of matrix metalloproteinase-1 activity by the soybean Bowman–Birk inhibitor. Biotechnol Lett 2004, 26:901-905.
• [9]Manuskiatti W, Maibach H: Hyaluronic acid and skin: wound healing and aging. Int J Dermatol 1996, 35(8):539-544.
• [10]Hsu M-F, Chiang B-H: Stimulating effects of Bacillus subtilis natto-fermented Radix astragali on hyaluronic acid production in human skin cells. J Ethnopharmacol 2009, 125:474-481.
• [11]Wang K-H, Lin R-D, Hsu F-L, Huang Y-H, Chang H-C, Huang C-Y, Lee M-H: Cosmetic applications of selected traditional Chinese herbal medicines. J Ethnopharmacol 2006, 106:353-359.
• [12]Irshad M, Chaudhuri PS: Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol 2002, 40:1233-1239.
• [13]Labat-Robert J, Fourtanier A, Boyer-Lafargue B, Robert L: Age dependent increase of elastase type protease activity in mouse skin effect of UV-irradiation. J Photochem Photobiol B 2000, 57:113-118.
• [14]Sumantran VN, Kulkarni AA, Harsulkar A, Wele A, Koppikar SJ, Chandwaskar R, Gaire V, Dalvi M, Wagh UV: Hyaluronidase and collagenase inhibitory activities of the herbal formulation Triphala guggulu. J Biosci 2007, 32(4):755-761.
• [15]Bizimenyera ES, Aderogba MA, Eloff JN, Swan GE: Potential of neuroprotective antioxidant-based therapeutics from Peltophorum africanum Sond. (Fabaceae). Afr J Trad CAM 2007, 4(1):99-106.
• [16]Van Wyk B-E, Gericke N: People’s plants: a guide to useful plants in southern Africa. Pretoria: Briza Publications; 2000.
• [17]Watt JM, Breyer-Brandwijk MG: The medicinal and poisonous plants of southern and eastern Africa. 2nd edition. London: Livingstone; 1962.
• [18]Kafua L, Kritzinger Q, Hussein A: Antifungal activity of Psychotria capensis leaf extracts [abstract]. S Afr J Bot 2009, 75(2):1.
• [19]Durling NE, Catchpole OJ, Grey JB, Webby RF, Mitchell KA, Foo LY, Perry NB: Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol-water mixtures. Food Chem 2007, 101:1417-1424.
• [20]Karim AA, Rajeev B, Alothman M: Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chem 2009, 115:785-788.
• [21]Kraunsoe JAE, Claridge TDW, Lowe G: Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry 1996, 35:9090-9096.
• [22]Moore S, Stein WH: Photometric ninhydrin method for use in the chromatography of amino acids. J Biol Chem 1948, 176:367-388.
• [23]Mandl I, MacLennan JD, Howes EL, DeBellis RH, Sohler A: Isolation and characterisation of proteinase and collagenase from CL. histolyticum. J Clin Invest 1953, 32:1323-1329.
• [24]Reissig JL, Strominger JL, Leloir LF: A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem 1955, 217:959-966.
• [25]Takahashi T, Ikegami-Kawai M, Okuda R, Suzuki K: A fluorimetric Morgan-Elson assay method for Hyaluronidase activity. Anal Biochem 2003, 322:257-263.
• [26]Re R, Pellegrinin N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999, 26:1231-1237.
• [27]Shapiro SS, Wilk MB: Analysis of variance test for normality (complete samples). Biometrika 1965, 52:591-611.
• [28]SAS Institute Inc: SAS/STAT User’s Guide, version 9. Cary: North Carolina: 1st printing, volume 2; 1999.
• [29]Glass GV, Peckham PD, Sanders JR: Consequences of failure to meet assumptions underlying the fixed effects analysis of variance and covariance. Rev Educ Res 1972, 42(3):237-288.
• [30]Tamburro AML: Elastin: molecular description and function. Int J Biochem Cell B 1999, 31:261-272.
• [31]Xu G-H, Kim Y-H, Chi S-W, Choo S-J, Ryoo I-J, Ahn J-S, Yoo I-D: Evaluation of human neutrophil elastase inhibitory effect of iridoid glycosides from Hedyotis diffusa. Bioorgan Med Chem Lett 2010, 20:513-515.
• [32]Wu S-F, Hwang T-L, Chen S-L, Wu C-C, Ohkoshi E, Lee K-H, Chang F-R, Wu Y-C: Bioactive components from the heartwood of Pterocarpus santalinus. Bioorgan Med Chem Lett 2011, 21:5630-5632.
• [33]Lee C-L, Liao Y-C, Hwang TL, Wu C-C, Chang F-R, Wu Y-C: Ixorapeptide I and ixorapeptide II, bioactive peptides isolated from Ixora coccinea. Bioorgan Med Chem Lett 2010, 20:7354-7357.
• [34]Chiang H-M, Lin T-J, Chiu C-Y, Chang C-W, Hsu K-C, Fan P-C, Wen K-C: Coffea arabica extract and its constituents prevent photoaging by suppressing MMPs expression and MAP kinase pathway. Food Chem Toxicol 2011, 49:309-318.
• [35]Sgariglia MA, Soberon JR, Cabanes AP, Sampietro DA, Vattuone MA: Anti-inflammatory properties of phenolic lactones isolated from Caesalpinia paraguariensis stem bark. J Ethnopharmacol 2013, 147:63-73.
• [36]Manaharan T, Teng LL, Appleton D, Ming CH, Masilamani T, Palanisamy UD: Antioxidant and antiglycemic potential of Peltophorum pterocarpum plant parts. Food Chem 2011, 129:1355-1361.
• [37]Pavagadhi S, Joseph GS, Jena BS: Antioxidant principles in Peltophorum ferrugineum flower extracts. Int J Food Prop 2012, 15:549-557.
• [38]Bahia MV, David JM, Rezende LC, Guedes MLS, David JP: A C-glucoside benzoic acid derivative from the leaves of Peltophorum dubium. Phytochem Lett 2010, 3:168-170.
• [39]Poullain C, Girard-Valenciennes E, Smadja J: Plants from reunion island: evaluation of their free radical scavenging and antioxidant activities. J Ethnopharmacol 2004, 95:19-26.
• [40]Arokiyaraj S, Sripriya N, Bhagya R, Radhika B, Prameela L, Udayaprakash NK: Phytochemical screening, antibacterial and free radical scavenging effects of Artemisia nilagirica, mimosa pudica and Clerodendrum siphonathus – an in-vitro study. Asian Pac J Trop Biomed 2012, 2(Suppl 2):S601-S604.
• [41]Jadeja RN, Thounaojam MC, Singh TB, Devkar RV, Ramachandran AV: Traditional uses, phytochemistry and pharmacology of Clerodendron glandulosum Coleb – a review. Asian Pac J Trop Med 2012, 5(1):1-6.
• [42]Adewusi EA, Moodley N, Steenkamp V: Antioxidant and acetylcholinesterase inhibitory activity of selected southern African medicinal plants. S Afr J Bot 2011, 77:638-644.
• [43]Steenkamp V, Grimmer H, Semano M, Gulumian M: Antioxidant and genotoxic properties of South African herbal extracts. Mutat Res 2005, 581:35-42.
• [44]Glasby JS: Dictionary of plants containing secondary metabolites. London: Taylor and Francis; 1991.
• [45]Nascimento NC, Fragoso V, Moura DJ, Romano e Silva AC, Fett-Neto AG, Saffi J: Antioxidant and antimutagenic effects of the crude folia extract and the alkaloid brachycerine of Psychotria brachyceras. Environ Mol Mutagen 2007, 48:728-734.
• [46]Fragoso V, Nascimento NC, Moura DJ: Antioxidant and antimutagenic properties of the monoterpene indole alkaloid psychollatine and the crude folia extract of Psychotria umbellata Vell. Toxicol in vitro 2008, 22:559-566.
• [47]Saha K, Lajis NH, Israf DA, Hamzaf AS, Khozirah S, Khamis S, Syahida A: Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J Ethnopharmacol 2004, 92:263-267.