期刊论文详细信息
BMC Infectious Diseases
NDM-1 Metallo-β-Lactamase and ArmA 16S rRNA methylase producing Providencia rettgeri clinical isolates in Nepal
Bharat M Pokhrel3  Teruo Kirikae1  Kayo Shimada1  Hiroshi Ohara2  Manoj K Sah3  Rajan K Dahal3  Tohru Miyoshi-Akiyama1  Tatsuya Tada1 
[1] Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan;Department of International Medical-Cooporation, National Center for Global Health and Medicine, Shinjuku, Tokyo Japan;Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal
关键词: Molecular epidemiology;    Providencia rettgeri;    16S rRNA methylase;    OXA-72;    NDM-1;   
Others  :  1134811
DOI  :  10.1186/1471-2334-14-56
 received in 2013-05-01, accepted in 2014-01-27,  发布年份 2014
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【 摘 要 】

Background

Drug-resistant Providencia rettgeri producing metallo-β-lactamase and 16S rRNA methylase has been reported in several countries. We analyzed P. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in Nepal.

Methods

Five clinical isolates of multidrug-resistant P. rettgeri were obtained in a hospital in Nepal. Antimicrobial susceptibilities were determined using the microdilution method and entire genomes were sequenced to determine drug-resistant genes. Epidemiological analysis was performed by pulsed-field gel electrophoresis.

Results

Four of the 5 isolates were resistant to carbapenems (imipenem and meropenem), with MICs ≥16 mg/L, with the remaining isolate showing intermediate resistance to imipenem, with an MIC of 2 mg/L and susceptibility to meropenem with an MIC ≤1 mg/L. All 5 isolates had blaVEB-1. Of the 4 carbapenem-resistant strains, 3 had blaNDM-1 and 1 had blaOXA-72. All isolates were highly resistant to aminoglycosides (MICs ≥1,024 mg/L) and harbored armA. As the result of pulsed-field gel electrophoresis pattern analysis in the 5 P. rettgeri isolates, 4 had identical PFGE patterns and the fifth showed 95.7% similarity.

Conclusions

This is the first report describing multidrug-resistant P. rettgeri strains harboring blaNDM-1 or blaOXA-72 and armA isolated from patients in Nepal.

【 授权许可】

   
2014 Tada et al.; licensee BioMed Central Ltd.

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