Cleavage site and Ectodomain of HA2 sub-unit sequence of three equine influenza virus isolated in Morocco

BMC Research Notes

Moulay Mustapha Ennaji³  Mehdi Elharrak¹  Nadia Touil²  Fathiah Zakham³  Mohamed Boukharta³ 

[1] Société de Produits biologiques et pharmaceutiques vétérinaires (Biopharma), Rabat, Morocco;Department of Biology, Instruction Military Hospital Med V Rabat, University Mohammed V Souissi, Rabat, Morocco;University Hassan II, Faculty of Sciences and Techniques, Mohammedia-Casablanca, Laboratory of Virology, Microbiology and Quality/ETB, Mohammedia BP 146, (20650), Morocco

关键词: Cleavage site;    Ectodomain (ED);    HA2 sequences;    Hemagglutinin (HA);    Equine influenza;   

Others  :  1131925
DOI  :  10.1186/1756-0500-7-448

 received in 2013-03-14, accepted in 2014-06-23,  发布年份 2014

【 摘 要 】

Background

The equine influenza (EI) is an infectious and contagious disease of the upper respiratory tract of horses. Two outbreaks were notified in Morocco during 1997 and 2004 respectively in Nador and Essaouira. The aims of the present study concern the amino acids sequences comparison with reference strain A/equine/Miami/1963(H3N8) of the HA2 subunit including the cleavage site of three equine influenza viruses (H3N8) isolated in Morocco: A/equine/Nador/1/1997(H3N8), A/equine/Essaouira/2/2004 (H3N8) and A/equine/Essaouira/3/2004 (H3N8).

Results

The obtained results demonstrated that the substitutions were located at Ectodomain (ED) and transmembrane domain (TD), and they have only one arginine in cleavage site (HA1-PEKQI-R³²⁹-GI-HA2). In the Ectodomain, the mutation N/154²/T deleted the NGT glycosylation site at position 154 for both strains A/equine/Essaouira/2/2004(H3N8) and A/equine/Essaouira/3/2004(H3N8). Except for mutation D/160²/Y of the A/equine/Nador/1/1997(H3N8) strain, the other mutations were involved in non conserved sites. While the transmembrane domain (TM) of the strain A/equine/Essaouira/3/2004(H3N8) exhibits a substitution at residue C/199²/F. For the A/equine/Nador/1/1997(H3N8) strain the HA2 shows a mutation at residue M/207²/L. Three Moroccan strains reveals a common substitution at the residue E/211²/Q located between transmembrane domain TM and the cytoplasmic domain (CD).

Conclusion

The given nature virulence of three Moroccan strains, the identified and reported mutations certainly played a permissive role of infection viral process.

【 授权许可】

   
2014 Boukharta et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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