High intralocus variability and interlocus recombination promote immunological diversity in a minimal major histocompatibility system

BMC Evolutionary Biology

Angela Bahr²  Camilla M Whittington¹  Anthony B Wilson³ 

[1]School of Biological Sciences, University of Sydney, Heydon-Laurence Building A08, Sydney 2006, NSW, Australia

[2]Institute of Medical Molecular Genetics, University of Zurich, Wagistrasse 12, Schlieren, 8952, Switzerland

[3]Department of Biology, Brooklyn College, 2900 Bedford Avenue, Brooklyn 11210, New York, USA

关键词: Syngnathidae;    Sexual selection;    Recombination;    Natural selection;    Major histocompatibility complex;    Gene conversion;   

Others  :  1121739
DOI  :  10.1186/s12862-014-0273-1

 received in 2014-08-14, accepted in 2014-12-12,  发布年份 2014

【 摘 要 】

Background

The genes of the major histocompatibility complex (MHC/MH) have attracted considerable scientific interest due to their exceptional levels of variability and important function as part of the adaptive immune system. Despite a large number of studies on MH class II diversity of both model and non-model organisms, most research has focused on patterns of genetic variability at individual loci, failing to capture the functional diversity of the biologically active dimeric molecule. Here, we take a systematic approach to the study of MH variation, analyzing patterns of genetic variation at MH class IIα and IIβ loci of the seahorse, which together form the immunologically active peptide binding cleft of the MH class II molecule.

Results

The seahorse carries a minimal class II system, consisting of single copies of both MH class IIα and IIβ, which are physically linked and inherited in a Mendelian fashion. Both genes are ubiquitously expressed and detectible in the brood pouch of male seahorses throughout pregnancy. Genetic variability of the two genes is high, dominated by non-synonymous variation concentrated in their peptide-binding regions. Coding variation outside these regions is negligible, a pattern thought to be driven by intra- and interlocus recombination. Despite the tight physical linkage of MH IIα and IIβ loci, recombination has produced novel composite alleles, increasing functional diversity at sites responsible for antigen recognition.

Conclusions

Antigen recognition by the adaptive immune system of the seahorse is enhanced by high variability at both MH class IIα and IIβ loci. Strong positive selection on sites involved in pathogen recognition, coupled with high levels of intra- and interlocus recombination, produce a patchwork pattern of genetic variation driven by genetic hitchhiking. Studies focusing on variation at individual MH loci may unintentionally overlook an important component of ecologically relevant variation.

【 授权许可】

   
2014 Wilson et al.; licensee BioMed Central.

【 图 表 】

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