Photosynthetic carbon from algal symbionts peaks during the latter stages of embryonic development in the salamander Ambystoma maculatum

BMC Research Notes

Robert W Sanders¹  Zaid M McKie-Krisberg¹  Erin R Graham¹ 

[1] Department of Biology, Temple University, 1900 N. 12th St., Philadelphia, PA 19122, USA

关键词: Carbon translocation;    Salamander;    Symbiosis;    Algae;   

Others  :  1125799
DOI  :  10.1186/1756-0500-7-764

 received in 2013-12-05, accepted in 2014-10-10,  发布年份 2014

【 摘 要 】

Background

It was recently discovered that symbiotic algae in the eggs of the salamander Ambystoma maculatum translocate fixed carbon from photosynthesis to developing embryos. Fixed carbon translocation was shown in embryos at one time point during development, however, it was unknown if fixed carbon translocation occurs throughout all developmental stages.

Findings

In this study, fixed carbon translocation was measured in salamander eggs at six time points over the latter half of development. Fixed carbon translocation did not occur until the middle tailbud portion of development (stages 26–30), and translocation was measured in 20% or less of eggs sampled. Peak carbon translocation occurred during the late tailbud phase of development (stages 31–35), where as much as 87% of eggs sampled showed translocation, and average percent translocation was 6.5%. During the final stages of development, fixed carbon translocation declined, and translocation was not detected in embryos five days prior to hatching.

Conclusions

The onset of fixed carbon translocation from Oophila to A. maculatum embryos during the second half of embryonic development is likely due to the corresponding settlement and concentration of Oophila in the inner egg envelope. In addition, carbon translocation ceases in late stage embryos as the inner egg envelope thins and ruptures in preparation for hatching.

【 授权许可】

   
2014 Graham et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Printz H: Chlorophyceae. In Die naturlichen Pflanzenfamilien [The Natural Plant Families]. Edited by Engler A, Prantl K. Leipzig, Germany: W. Engelmann; 1928. Vol 3, pp 1–463
• [2]Pinder A, Friet S: Oxygen transport in egg masses of the amphibians Rana sylvatica and Ambystoma maculatum: convection, diffusion and oxygen production by algae. J Exp Biol 1994, 197(1):17-30.
• [3]Valls JH, Mills NE: Intermittent hypoxia in eggs of Ambystoma maculatum: embryonic development and egg capsule conductance. J Exp Biol 2007, 210(14):2430-2435.
• [4]Gilbert PW: Observations on the eggs of Ambystoma maculatum with especial reference to the green algae found within the egg envelopes. Ecology 1942, 23(2):215-227.
• [5]Gilbert PW: The alga-egg relationship in Ambystoma maculatum, a case of symbiosis. Ecology 1944, 25(3):366-369.
• [6]Tattersall GJ, Spiegelaar N: Embryonic motility and hatching success of Ambystoma maculatum are influenced by a symbiotic alga. Can J Zool 2008, 86(11):1289-1298.
• [7]Graham ER, Fay SA, Davey A, Sanders RW: Intracapsular algae provide fixed carbon to developing embryos of the salamander Ambystoma maculatum. J Exp Biol 2013, 216(Pt 3):452-459.
• [8]Bordzilovskaya NP, Dettlaff TA, Duhon ST, Malacinski GM: Developmental-Stage Series of Axolotl Embryos. In In Developmental Biology of the Axolotl. Edited by Malacinski GM, Armstrong JB. New York: Oxford University Press; 1989:201-219.
• [9]Harrison RG: Organization and Development of the Embryo. Edited by Wilens S. New Haven,CT: Yale University Press; 1969:44-66.
• [10]Kerney R, Kim E, Hangarter RP, Heiss AA, Bishop CD, Hall BK: Intracellular invasion of green algae in a salamander host. Proc Natl Acad Sci U S A 2011, 108(16):6497.